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ZnT7 Polyclonal antibody

ZnT7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13966-1-AP

Synonyms

ZnT 7, SLC30A7, Znt-like transporter 2, ZNTL2, ZnT-7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZnT7 Polyclonal antibody (13966-1-AP)

    WB analysis using 13966-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13966-1-AP (ZnT7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ZnT7 Polyclonal antibody (13966-1-AP)

    IHC staining of human lung cancer using 13966-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13966-1-AP (ZnT7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ZnT7 Polyclonal antibody (13966-1-AP)

    IHC staining of human lung cancer using 13966-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13966-1-AP (ZnT7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ZnT7 Polyclonal antibody (13966-1-AP)

    IHC staining of human lung cancer using 13966-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13966-1-AP (ZnT7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ZnT7 Polyclonal antibody (13966-1-AP)

    IHC staining of human lung cancer using 13966-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13966-1-AP (ZnT7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ZnT7 Polyclonal antibody (13966-1-AP)

    IF Staining of A431 using 13966-1-AP

    Immunofluorescent analysis of A431 cells, using SLC30A7 antibody 13966-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inC2C12 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13966-1-AP targets ZnT7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZnT7 fusion protein Ag4957
Full Name solute carrier family 30 (zinc transporter), member 7
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC064692
Gene Symbol SLC30A7
Gene ID (NCBI) 148867
RRIDAB_2189776
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZnT7 antibody 13966-1-APDownload protocol
IHC protocol for ZnT7 antibody 13966-1-APDownload protocol
IF protocol for ZnT7 antibody 13966-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Acta Neuropathol Commun

Deregulation of subcellular biometal homeostasis through loss of the metal transporter, Zip7, in a childhood neurodegenerative disorder.

Authors - Alexandra Grubman
View Article
mouseWB

Metallomics

Deregulation of biometal homeostasis: the missing link for neuronal ceroid lipofuscinoses?

Authors - Alexandra Grubman
View Article
ratWB

Am J Physiol Gastrointest Liver Physiol

Zinc deficiency mediates alcohol-induced apoptotic cell death in the liver of rats through activating ER and mitochondrial cell death pathways.

Authors - Qian Sun
View Article
mouseWB

Am J Physiol Gastrointest Liver Physiol

Dysregulation of hepatic zinc transporters in a mouse model of alcoholic liver disease.

Authors - Qian Sun
View Article
chickenWB

Front Physiol

Organic zinc with moderate chelation strength enhances zinc absorption in the small intestine and expression of related transporters in the duodenum of broilers

Authors - Yun Hu
View Article
chickenWB

J Anim Sci

Zinc proteinate with moderate chelation strength enhances zinc absorption by upregulating the expression of zinc and amino acid transporters in primary cultured duodenal epithelial cells of broiler embryos

Authors - Yun Hu
View Article