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FATP4 Polyclonal antibody

Name: FATP4 Polyclonal antibody
Brand: Proteintech
SKU: 11013-1-AP

FATP4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11013-1-AP

Synonyms

ACSVL4, FATP 4, FATP4, Fatty acid transport protein 4, SLC27A4

Validation Data Gallery

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AllWBIPIHC

WB analysis of HepG2 using 11013-1-AP
WB result of FATP4 antibody (11013-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-FATP4 transfected HepG2 cells.
WB analysis of human colon using 11013-1-AP
human colon tissue were subjected to SDS PAGE followed by western blot with 11013-1-AP (FATP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis using 11013-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11013-1-AP (FATP4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 11013-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11013-1-AP (FATP4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of SMMC-7721 using 11013-1-AP
SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 11013-1-AP (FATP4 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 11013-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 11013-1-AP (FATP4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 11013-1-AP
IP result of anti-FATP4 (IP:11013-1-AP, 4ug; Detection:11013-1-AP 1:500) with HepG2 cells lysate 1600ug.
IHC staining of human ovary tumor using 11013-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11013-1-AP (FATP4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 11013-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11013-1-AP (FATP4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human ovary tumor using 11013-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11013-1-AP (FATP4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 11013-1-AP

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Tested Applications

Positive WB detected in	HepG2 cells, mouse liver tissue, human colon tissue, human heart tissue, SMMC-7721 cells, mouse colon tissue, rat colon tissue, rat liver tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 10 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

11013-1-AP targets FATP4 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	FATP4 fusion protein Ag1490
Full Name	solute carrier family 27 (fatty acid transporter), member 4
Calculated Molecular Weight	72 kDa
Observed Molecular Weight	70 kDa
GenBank Accession Number	BC009959
Gene Symbol	FATP4/SLC27A4
Gene ID (NCBI)	10999
RRID	AB_2190637
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for FATP4 antibody 11013-1-AP	Download protocol
IHC protocol for FATP4 antibody 11013-1-AP	Download protocol
IP protocol for FATP4 antibody 11013-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	Sci Rep PMID: 28304381 Authors - Wenjing Li View Article
human	WB	Front Immunol Cathelicidin-WA Facilitated Intestinal Fatty Acid Absorption Through Enhancing PPAR-γ Dependent Barrier Function. Authors - Xin Zong View Article
mouse	WB	iScience Celastrol inhibits intestinal lipid absorption by reprofiling the gut microbiota to attenuate high-fat diet-induced obesity. Authors - Hu Hua View Article
	WB	Int J Mol Sci Nano-Zn Increased Zn Accumulation and Triglyceride Content by Up-Regulating Lipogenesis in Freshwater Teleost, Yellow Catfish Pelteobagrus fulvidraco. Authors - Shi-Cheng Ling View Article
mouse	IHC	Biochim Biophys Acta Uninephrectomy augments the effects of high fat diet induced obesity on gene expression in mouse kidney. Authors - Zhibo Gai View Article
mouse,pig	WB	Cells LPS Inhibits Fatty Acid Absorption in Enterocytes through TNF-α Secreted by Macrophages. Authors - Heyuan Liu View Article

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Research Area

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FATP4 Polyclonal antibody

FATP4 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis of HepG2 using 11013-1-AP

WB analysis of human colon using 11013-1-AP

WB analysis using 11013-1-AP

WB analysis using 11013-1-AP

WB analysis of SMMC-7721 using 11013-1-AP

WB analysis of human heart using 11013-1-AP

IP experiment of HepG2 using 11013-1-AP

IHC staining of human ovary tumor using 11013-1-AP

IHC staining of human ovary tumor using 11013-1-AP

IHC staining of human ovary tumor using 11013-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

PMID: 28304381

Cathelicidin-WA Facilitated Intestinal Fatty Acid Absorption Through Enhancing PPAR-γ Dependent Barrier Function.

Celastrol inhibits intestinal lipid absorption by reprofiling the gut microbiota to attenuate high-fat diet-induced obesity.

Nano-Zn Increased Zn Accumulation and Triglyceride Content by Up-Regulating Lipogenesis in Freshwater Teleost, Yellow Catfish Pelteobagrus fulvidraco.

Uninephrectomy augments the effects of high fat diet induced obesity on gene expression in mouse kidney.

LPS Inhibits Fatty Acid Absorption in Enterocytes through TNF-α Secreted by Macrophages.