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  • Featured Product
  • KD/KO Validated

DSS1 Polyclonal antibody

DSS1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13639-1-AP

Synonyms

DSS1, ECD, SEM1, SHFD1, Shfdg1, SHFM1, SHSF1

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human skin cancer tissue using DSS1 Polyclonal antibody (13639-1-AP)

    IHC staining of human skin cancer using 13639-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13639-1-AP (DSS1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using DSS1 Polyclonal antibody (13639-1-AP)

    IHC staining of human skin cancer using 13639-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13639-1-AP (DSS1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using DSS1 Polyclonal antibody (13639-1-AP)

    IHC staining of human cervical cancer using 13639-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13639-1-AP (DSS1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using DSS1 Polyclonal antibody (13639-1-AP)

    IHC staining of human cervical cancer using 13639-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13639-1-AP (DSS1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using DSS1 Polyclonal antibody (13639-1-AP)

    IHC staining of human placenta using 13639-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13639-1-AP (DSS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using DSS1 Polyclonal antibody (13639-1-AP)

    IHC staining of human placenta using 13639-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13639-1-AP (DSS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inhuman skin cancer tissue, human cervical cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13639-1-AP targets DSS1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DSS1 fusion protein Ag4573
Full Name split hand/foot malformation (ectrodactyly) type 1
Calculated Molecular Weight 70 aa, 8 kDa
GenBank Accession NumberBC032782
Gene Symbol DSS1
Gene ID (NCBI) 7979
RRIDAB_2254633
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for DSS1 antibody 13639-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2.

Authors - Vaibhav Bhatia
  • KD Validated
View Article
humanWB, IF

EMBO J

The proteasomal de-ubiquitinating enzyme POH1 promotes the double-strand DNA break response.

Authors - Laura R Butler
View Article

J Med Genet

Absent expression of the osteoblast-specific maternally imprinted genes, DLX5 and DLX6, causes split hand/split foot malformation type I.

Authors - Sawitree Rattanasopha
View Article
humanIHC

Lab Invest

Increased chemosensitivity via BRCA2-independent DNA damage in DSS1- and PCID2-depleted breast carcinomas.

Authors - Naomi Gondo
  • KD Validated
View Article
humanWB

BMC Cancer

Breast cancers with high DSS1 expression that potentially maintains BRCA2 stability have poor prognosis in the relapse-free survival.

Authors - Andri Rezano
View Article
humanWB

Protein Cell

DSSylation, a novel protein modification targets proteins induced by oxidative stress, and facilitates their degradation in cells.

Authors - Yinghao Zhang
View Article