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  • Featured Product
  • KD/KO Validated

SHC4 Polyclonal antibody

SHC4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12641-1-AP

Synonyms

SHC transforming protein 4, SH2 domain protein C4, RaLP, Rai-like protein, Rai like protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SHC4 Polyclonal antibody (12641-1-AP)

    WB analysis using 12641-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SHC4 Polyclonal antibody (12641-1-AP)

    WB analysis using 12641-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using SHC4 Polyclonal antibody (12641-1-AP)

    WB analysis of human brain using 12641-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SHC4 Polyclonal antibody (12641-1-AP)

    WB analysis of HepG2 using 12641-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using SHC4 Polyclonal antibody (12641-1-AP)

    WB analysis of human skeletal muscle using 12641-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using SHC4 Polyclonal antibody (12641-1-AP)

    WB analysis of A375 using 12641-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat brain tissue using SHC4 Polyclonal antibody (12641-1-AP)

    IHC staining of rat brain using 12641-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12641-1-AP (SHC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SHC4 Polyclonal antibody (12641-1-AP)

    IHC staining of mouse brain using 12641-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12641-1-AP (SHC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using SHC4 Polyclonal antibody (12641-1-AP)

    IHC staining of rat brain using 12641-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12641-1-AP (SHC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using SHC4 Polyclonal antibody (12641-1-AP)

    IF Staining of SH-SY5Y using 12641-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using SHC4 antibody (12641-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SHC4 Polyclonal antibody (12641-1-AP)

    IF Staining of HepG2 using 12641-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SHC4 antibody (12641-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inSH-SY5Y cells, human brain tissue, HepG2 cells, human skeletal muscle tissue, mouse brain tissue, A375 cells, rat brain tissue
Positive IHC detected inrat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12641-1-AP targets SHC4 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHC4 fusion protein Ag3336
Full Name SHC (Src homology 2 domain containing) family, member 4
Calculated Molecular Weight 630 aa, 69 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC033907
Gene Symbol SHC4
Gene ID (NCBI) 399694
RRIDAB_10643243
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SHC4 antibody 12641-1-APDownload protocol
IHC protocol for SHC4 antibody 12641-1-APDownload protocol
IF protocol for SHC4 antibody 12641-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Clin Sci (Lond)

Down-regulation of the insulin signaling pathway by SHC may correlate with congenital heart disease in Chinese populations.

Authors - Zhiling Luo
View Article
humanIHC,IF

PeerJ

A new 12-gene diagnostic biomarker signature of melanoma revealed by integrated microarray analysis.

Authors - Liu Wanting W
View Article
mouse,humanWB,IHC,IF,CoIP

Cancer Lett

SHC4 orchestrates β-catenin pathway-mediated metastasis in triple-negative breast cancer by promoting Src kinase autophosphorylation

Authors - Wenjing Zhong
  • KD Validated
View Article