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  • Featured Product
  • KD/KO Validated

SHC1 Polyclonal antibody

SHC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12496-1-AP

Synonyms

SHC, SH2 domain protein C1, SHC-transforming protein A, SHC-transforming protein 3, SHC-transforming protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SHC Polyclonal antibody (12496-1-AP)

    WB analysis using 12496-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12496-1-AP (SHC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SHC Polyclonal antibody (12496-1-AP)

    WB analysis of HeLa using 12496-1-AP

    WB result of SHC antibody (12496-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-SHC transfected HeLa cells.

  • Western Blot (WB) analysis of HeLa cells using SHC Polyclonal antibody (12496-1-AP)

    WB analysis of HeLa using 12496-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12496-1-AP (SHC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using SHC1 Polyclonal antibody (12496-1-AP)

    IHC staining of human stomach using 12496-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12496-1-AP (SHC antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SHC Polyclonal antibody (12496-1-AP)

    IF Staining of HeLa using 12496-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SHC antibody (12496-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SHC Polyclonal antibody (12496-1-AP)

    IF Staining of HepG2 using 12496-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SHC antibody (12496-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, A431 cells, C6 cells, NIH/3T3 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12496-1-AP targets SHC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHC1 fusion protein Ag3165
Full Name SHC (Src homology 2 domain containing) transforming protein 1
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 66 kDa, 52 kDa, 46 kDa
GenBank Accession NumberBC014158
Gene Symbol SHC1
Gene ID (NCBI) 6464
RRIDAB_2187889
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The proto-oncogene SHC family consists of SHCA, SHCB, SHCC, and SHCD4 gene members, which can encode 7 kinds of cohesive molecules. SHC family is widely expressed and functional. The SHC1 gene is located in zone 1, region 2 of human chromosome 1. SHC1 is believed to be involved in inhibiting neuronal apoptosis and regulating the receptor tyrosine kinase pathway. SHC1 sensitizes cancer cells to the 8-Cl-cAMP treatment.

Protocols

Product Specific Protocols
WB protocol for SHC1 antibody 12496-1-APDownload protocol
IHC protocol for SHC1 antibody 12496-1-APDownload protocol
IF protocol for SHC1 antibody 12496-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Exp Cell Res

LRTM1 promotes the differentiation of myoblast cells by negatively regulating the FGFR1 signaling pathway.

Authors - Hao-Ke Li
View Article
humanWB,IF

Sci Adv

HPIP is an essential scaffolding protein running through the EGFR-RAS-ERK pathway and drives tumorigenesis

Authors - Qiwei Jiang
View Article