Bif-1 Polyclonal antibody
Bif-1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 15422-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse kidney tissue, HEK-293 cells, human placenta tissue, mouse heart tissue, mouse placenta tissue |
| Positive IHC detected in | mouse kidney tissue, human kidney tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
15422-1-AP targets Bif-1 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Bif-1 fusion protein Ag7710 |
| Full Name | SH3-domain GRB2-like endophilin B1 |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 41 kDa |
| GenBank Accession Number | BC007455 |
| Gene Symbol | Bif-1 |
| Gene ID (NCBI) | 51100 |
| RRID | AB_2254564 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SH3GLB1 also known as Bif-1, contains an N-terminal domain, a central coiled-coil region, and a C-terminal SH3 domain, but shares no significant homology with members of the Bcl-2 family. SH3GLB1 is involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation (PubMed:16227588).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Bif-1 antibody 15422-1-AP | Download protocol |
| IHC protocol for Bif-1 antibody 15422-1-AP | Download protocol |
| IF protocol for Bif-1 antibody 15422-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biomed Mater Nano implant surface triggers autophagy through membrane curvature distortion to regulate the osteogenic differentiation | ||
Eur J Pharmacol SUMO2-mediated SUMOylation of SH3GLB1 promotes ionizing radiation-induced hypertrophic cardiomyopathy through mitophagy activation. | ||
J Exp Clin Cancer Res SH3GLB1-related autophagy mediates mitochondrial metabolism to acquire resistance against temozolomide in glioblastoma. | ||
Oncotarget Autophagy-related gene expression is an independent prognostic indicator of glioma. | ||
