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Serpin C1/Antithrombin III Polyclonal antibody

Serpin C1/Antithrombin III Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16414-1-AP

Synonyms

Antithrombin III, Serpin C1, SERPINC1, PRO0309, ATIII

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human plasma using Serpin C1/Antithrombin III Polyclonal antibody (16414-1-AP)

    WB analysis of human plasma using 16414-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 16414-1-AP (Antithrombin III antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using Serpin C1/Antithrombin III Polyclonal antibody (16414-1-AP)

    IHC staining of human liver using 16414-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16414-1-AP (Antithrombin III antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Serpin C1/Antithrombin III Polyclonal antibody (16414-1-AP)

    IHC staining of human liver using 16414-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16414-1-AP (Antithrombin III antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Serpin C1/Antithrombin III Polyclonal antibody (16414-1-AP)

    IF Staining of HepG2 using 16414-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16414-1-AP (Antithrombin III antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman blood tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16414-1-AP targets Serpin C1/Antithrombin III in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Serpin C1/Antithrombin III fusion protein Ag9565
Full Name serpin peptidase inhibitor, clade C (antithrombin), member 1
Calculated Molecular Weight 464 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC022309
Gene Symbol Serpin C1/Antithrombin III
Gene ID (NCBI) 462
RRIDAB_2878255
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERPINC1, also named Antithrombin III or Antithrombin, is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency.

Protocols

Product Specific Protocols
WB protocol for Serpin C1/Antithrombin III antibody 16414-1-APDownload protocol
IHC protocol for Serpin C1/Antithrombin III antibody 16414-1-APDownload protocol
IF protocol for Serpin C1/Antithrombin III antibody 16414-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Rep

Molecular Camouflage of Plasmodium falciparum Merozoites by Binding of Host Vitronectin to P47 Fragment of SERA5.

Authors - Takahiro Tougan
View Article