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Kallistatin Polyclonal antibody

Kallistatin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11430-2-AP

Synonyms

Serpin A4, PI 4, Peptidase inhibitor 4, KST, KLST

Validation Data Gallery

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  • Western Blot (WB) analysis of human spleen tissue using Kallistatin Polyclonal antibody (11430-2-AP)

    WB analysis of human spleen using 11430-2-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 11430-2-AP (Kallistatin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human plasma using Kallistatin Polyclonal antibody (11430-2-AP)

    WB analysis of human plasma using 11430-2-AP

    human serum were subjected to SDS PAGE followed by western blot with 11430-2-AP (Kallistatin antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Kallistatin Polyclonal antibody (11430-2-AP)

    IHC staining of human pancreas cancer using 11430-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11430-2-AP (Kallistatin antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HCT 116 cells using Kallistatin Polyclonal antibody (11430-2-AP)

    IF Staining of HCT 116 using 11430-2-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using Kallistatin antibody (11430-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inhuman spleen tissue, human plasma tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHCT 116 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11430-2-AP targets Kallistatin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Kallistatin fusion protein Ag1962
Full Name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC014992
Gene Symbol Kallistatin
Gene ID (NCBI) 5267
RRIDAB_2185735
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Kallistatin antibody 11430-2-APDownload protocol
IHC protocol for Kallistatin antibody 11430-2-APDownload protocol
IF protocol for Kallistatin antibody 11430-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Diabetes

Pathogenic Role of Diabetes-induced Overexpression of Kallistatin in Corneal Wound Healing Deficiency through Inhibition of Canonical Wnt Signaling.

Authors - Wentao Liang
View Article