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SEC23IP Polyclonal antibody

SEC23IP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20892-1-AP

Synonyms

SEC23-interacting protein, p125, MSTP053

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEC23IP Polyclonal antibody (20892-1-AP)

    WB analysis using 20892-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20892-1-AP (SEC23IP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using SEC23IP Polyclonal antibody (20892-1-AP)

    WB analysis of mouse kidney using 20892-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20892-1-AP (SEC23IP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using SEC23IP Polyclonal antibody (20892-1-AP)

    IP experiment of HeLa using 20892-1-AP

    IP result of anti-SEC23IP (IP:20892-1-AP, 4ug; Detection:20892-1-AP 1:1000) with HeLa cells lysate 3600ug.

  • Immunohistochemistry (IHC) staining of human heart tissue using SEC23IP Polyclonal antibody (20892-1-AP)

    IHC staining of human heart using 20892-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 20892-1-AP (SEC23IP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using SEC23IP Polyclonal antibody (20892-1-AP)

    IHC staining of human heart using 20892-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 20892-1-AP (SEC23IP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using SEC23IP Polyclonal antibody (20892-1-AP)

    IHC staining of human liver using 20892-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 20892-1-AP (SEC23IP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using SEC23IP Polyclonal antibody (20892-1-AP)

    IHC staining of human liver using 20892-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 20892-1-AP (SEC23IP antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SEC23IP Polyclonal antibody (20892-1-AP)

    IF Staining of HeLa using 20892-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SEC23IP antibody (20892-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, mouse kidney tissue, HeLa cells, U-251 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman heart tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20892-1-AP targets SEC23IP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEC23IP fusion protein Ag14916
Full Name SEC23 interacting protein
Calculated Molecular Weight 1000 aa, 111 kDa
Observed Molecular Weight 125 kDa
GenBank Accession NumberBC063800
Gene Symbol SEC23IP
Gene ID (NCBI) 11196
RRIDAB_10896458
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEC23-interacting protein (SEC23IP), also known as p125, is a member of the phosphatidic acid preferring-phospholipase A1 family. SEC23IP interacts with Sec23, a coat component of COPII vesicles which are involved in protein export from the endoplasmic reticulum (ER). SEC23IP localizes to ER exit sites and participates in the organization of this compartment (PMID: 15623529). It may have a role in the early stage of the secretory pathway (PMID: 10400679).

Protocols

Product Specific Protocols
WB protocol for SEC23IP antibody 20892-1-APDownload protocol
IHC protocol for SEC23IP antibody 20892-1-APDownload protocol
IF protocol for SEC23IP antibody 20892-1-APDownload protocol
IP protocol for SEC23IP antibody 20892-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Anal Chem

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Authors - Hongwei Chu
View Article
humanIF

J Cell Biol

Small GTPase ActIvitY ANalyzing (SAIYAN) system: A method to detect GTPase activation in living cells

Authors - Miharu Maeda
View Article