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SDCCAG10 Polyclonal antibody

SDCCAG10 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16502-1-AP

Synonyms

Probable inactive peptidyl-prolyl cis-trans isomerase CWC27 homolog, PPIase CWC27, NY CO 10, CWC27, Antigen NY-CO-10

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using SDCCAG10 Polyclonal antibody (16502-1-AP)

    WB analysis of HepG2 using 16502-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16502-1-AP (SDCCAG10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human colon tissue using SDCCAG10 Polyclonal antibody (16502-1-AP)

    WB analysis of human colon using 16502-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 16502-1-AP (SDCCAG10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SDCCAG10 Polyclonal antibody (16502-1-AP)

    WB analysis of HeLa using 16502-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16502-1-AP (SDCCAG10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using SDCCAG10 Polyclonal antibody (16502-1-AP)

    IP experiment of HepG2 using 16502-1-AP

    IP result of anti-SDCCAG10 (IP:16502-1-AP, 4ug; Detection:16502-1-AP 1:500) with HepG2 cells lysate 2400ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SDCCAG10 Polyclonal antibody (16502-1-AP)

    IF Staining of HepG2 using 16502-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16502-1-AP (SDCCAG10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, human colon tissue
Positive IP detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16502-1-AP targets SDCCAG10 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SDCCAG10 fusion protein Ag9671
Full Name serologically defined colon cancer antigen 10
Calculated Molecular Weight472aa,54 kDa; 291aa,33 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC012117
Gene Symbol SDCCAG10
Gene ID (NCBI) 10283
RRIDAB_1851625
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SDCCAG10 antibody 16502-1-APDownload protocol
IF protocol for SDCCAG10 antibody 16502-1-APDownload protocol
IP protocol for SDCCAG10 antibody 16502-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols