• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Mammaglobin A Polyclonal antibody

Mammaglobin A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23501-1-AP

Synonyms

hMAM, Mammaglobin 1, Mammaglobin A, MGB1, SCGB2A2, UGB2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of MCF-7 cells using Mammaglobin A Polyclonal antibody (23501-1-AP)

    WB analysis of MCF-7 using 23501-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 23501-1-AP (Mammaglobin A antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Mammaglobin A Polyclonal antibody (23501-1-AP)

    IHC staining of human breast cancer using 23501-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 23501-1-AP (Mammaglobin A antibody at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Mammaglobin A Polyclonal antibody (23501-1-AP)

    IHC staining of human breast cancer using 23501-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 23501-1-AP (Mammaglobin A antibody at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Mammaglobin A Polyclonal antibody (23501-1-AP)

    IHC staining of human breast cancer using 23501-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 23501-1-AP (Mammaglobin A antibody at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Mammaglobin A Polyclonal antibody (23501-1-AP)

    IHC staining of human breast cancer using 23501-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 23501-1-AP (Mammaglobin A antibody at dilution of 1:50.

Tested Applications

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

23501-1-AP targets Mammaglobin A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Mammaglobin A fusion protein Ag20122
Full Name secretoglobin, family 2A, member 2
Calculated Molecular Weight 120 aa, 13 kDa
Observed Molecular Weight 6-10 kDa
GenBank Accession NumberBC067220
Gene Symbol Mammaglobin A
Gene ID (NCBI) 4250
RRIDAB_2879289
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SCGB2A2, also known as mammaglobin A, is a member of the secretoglobin superfamily. It is a breast cancer-associated antigen almost exclusively over-expressed in primary and metastatic human breast cancers, making it a specific molecular marker and a potential therapeutic target for breast cancer. SCGB2A2 is a 93-amino acid protein with a calculated molecular weight of 10.5 kDa. This polyclonal antibody is raised against the C-terminal 71 amino acids of the protein encoded by a cDNA (MGC:71974; BC067220).

Protocols

Product Specific Protocols
WB protocol for Mammaglobin A antibody 23501-1-APDownload protocol
IHC protocol for Mammaglobin A antibody 23501-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Mol Med Rep

Immunolocalization of MMP9 and MMP2 in osteolytic metastasis originating from MDA-MB-231 human breast cancer cells.

Authors - Bo Liu
View Article