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- KD/KO Validated
SACM1L Polyclonal antibody
SACM1L Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 13033-1-AP
Synonyms
Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | human kidney tissue, A549 cells, mouse kidney tissue, mouse lung tissue, human brain tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human brain tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 6 publications below |
| WB | See 15 publications below |
| IF | See 6 publications below |
| IP | See 1 publications below |
Product Information
13033-1-AP targets SACM1L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SACM1L fusion protein Ag3682 |
| Full Name | SAC1 suppressor of actin mutations 1-like (yeast) |
| Calculated Molecular Weight | 587 aa, 67 kDa |
| Observed Molecular Weight | 60-67 kDa |
| GenBank Accession Number | BC016559 |
| Gene Symbol | SACM1L |
| Gene ID (NCBI) | 22908 |
| RRID | AB_2301284 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SACM1L(Suppressor of actin mutations 1-like protein) is also named KIAA0851. It is a phosphoinositide phosphatase that hydrolyzes PtdIns3P and PtdIns4P and has low activity towards PtdIns(3,5)P2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SACM1L antibody 13033-1-AP | Download protocol |
| IHC protocol for SACM1L antibody 13033-1-AP | Download protocol |
| IF protocol for SACM1L antibody 13033-1-AP | Download protocol |
| IP protocol for SACM1L antibody 13033-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Res Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity | ||
Cell Host Microbe Global mapping of Salmonella enterica-host protein-protein interactions during infection. | ||
Neuron An ER Assembly Line of AMPA-Receptors Controls Excitatory Neurotransmission and Its Plasticity. | ||
Autophagy PtdIns4P restriction by hydrolase SAC1 decides specific fusion of autophagosomes with lysosomes.
| ||
Proc Natl Acad Sci U S A Addiction to Golgi-resident PI4P synthesis in chromosome 1q21.3-amplified lung adenocarcinoma cells. | ||
J Cell Biol Sensing of nutrients by CPT1C controls SAC1 activity to regulate AMPA receptor trafficking. |
