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RXRG Polyclonal antibody

RXRG Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, PLA, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11129-1-AP

Synonyms

NR2B3, Retinoid X receptor gamma, retinoid X receptor, gamma, RXRC, RXRG

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using RXRG Polyclonal antibody (11129-1-AP)

    WB analysis of mouse heart using 11129-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 11129-1-AP (RXRG Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of mouse ovary tissue using RXRG Polyclonal antibody (11129-1-AP)

    WB analysis of mouse ovary using 11129-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11129-1-AP (RXRG Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using RXRG Polyclonal antibody (11129-1-AP)

    WB analysis of Jurkat using 11129-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11129-1-AP (RXRG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat eye tissue using RXRG Polyclonal antibody (11129-1-AP)

    IHC staining of rat eye using 11129-1-AP

    Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 11129-1-AP (RXRG antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat eye tissue using RXRG Polyclonal antibody (11129-1-AP)

    IHC staining of rat eye using 11129-1-AP

    Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 11129-1-AP (RXRG antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using RXRG Polyclonal antibody (11129-1-AP)

    IHC staining of mouse skeletal muscle using 11129-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11129-1-AP (RXRG antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using RXRG Polyclonal antibody (11129-1-AP)

    IHC staining of mouse skeletal muscle using 11129-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11129-1-AP (RXRG antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse heart tissue, Jurkat cells, mouse ovary tissue
Positive IHC detected inrat eye tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11129-1-AP targets RXRG in WB, IF, PLA, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RXRG fusion protein Ag1611
Full Name retinoid X receptor, gamma
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 51-55 kDa
GenBank Accession NumberBC012063
Gene Symbol RXRG
Gene ID (NCBI) 6258
RRIDAB_2182635
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RXRG, also named as, Retinoic acid receptor RXR-gamma, is a 463 amino acid protein, which localize in the nucleus and may form homodimer. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5.

Protocols

Product Specific Protocols
WB protocol for RXRG antibody 11129-1-APDownload protocol
IHC protocol for RXRG antibody 11129-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mousePLA

Aging (Albany NY)

Cholecalciferol (vitamin D3) has a direct protective activity against interleukin 6-induced atrophy in C2C12 myotubes.

Authors - Maraiza Alves Teixeira
View Article
ratIF

Am J Physiol Lung Cell Mol Physiol

Inhaled vitamin A is more effective than intramuscular dosing in mitigating hyperoxia-induced lung injury in a neonatal rat model of bronchopulmonary dysplasia.

Authors - Craig A Gelfand
View Article
humanWB

Cells

EGFR/ErbB Inhibition Promotes OPC Maturation up to Axon Engagement by Co-Regulating PIP2 and MBP.

Authors - Emanuela Nocita
View Article