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RXRB Polyclonal antibody

RXRB Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14684-1-AP

Synonyms

DAUDI6, H 2RIIBP, NR2B2, RCoR 1, Retinoid X receptor beta, retinoid X receptor, beta, RXRB

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using RXRB Polyclonal antibody (14684-1-AP)

    WB analysis of NIH/3T3 using 14684-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14684-1-AP (RXRB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using RXRB Polyclonal antibody (14684-1-AP)

    WB analysis of MCF-7 using 14684-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 14684-1-AP (RXRB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using RXRB Polyclonal antibody (14684-1-AP)

    IP experiment of MCF-7 using 14684-1-AP

    IP result of anti-RXRB (IP:14684-1-AP, 4ug; Detection:14684-1-AP 1:500) with MCF-7 cells lysate 1200ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RXRB Polyclonal antibody (14684-1-AP)

    IHC staining of human colon cancer using 14684-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14684-1-AP (RXRB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RXRB Polyclonal antibody (14684-1-AP)

    IHC staining of human colon cancer using 14684-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14684-1-AP (RXRB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inNIH/3T3 cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

14684-1-AP targets RXRB in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RXRB fusion protein Ag0263
Full Name retinoid X receptor, beta
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57 kDa, 70 kDa
GenBank Accession NumberBC001167
Gene Symbol RXRB
Gene ID (NCBI) 6257
RRIDAB_10858228
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Retinoid X Receptor beta (RXRB) is a member of the retinoid X receptor (RXR) family of nuclear receptors which are involved in mediating the effects of retinoic acid. RXRB heterodimerizes with other nuclear hormone receptors, such as RAR, the thyroid hormone receptor and the vitamin D3 receptor, to enhance binding to their cognate response elements. (PMID: 8257090)

Protocols

Product Specific Protocols
WB protocol for RXRB antibody 14684-1-APDownload protocol
IHC protocol for RXRB antibody 14684-1-APDownload protocol
IP protocol for RXRB antibody 14684-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

Am J Physiol Lung Cell Mol Physiol

Inhaled vitamin A is more effective than intramuscular dosing in mitigating hyperoxia-induced lung injury in a neonatal rat model of bronchopulmonary dysplasia.

Authors - Craig A Gelfand
View Article