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  • Featured Product
  • KD/KO Validated

RTKN2 Polyclonal antibody

RTKN2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17458-1-AP

Synonyms

Rhotekin-2, rhotekin 2, PLEKHK1, Pleckstrin homology domain-containing family K member 1, PH domain-containing family K member 1

Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using RTKN2 Polyclonal antibody (17458-1-AP)

    WB analysis of HL-60 using 17458-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 17458-1-AP (RTKN2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lymph tissue using RTKN2 Polyclonal antibody (17458-1-AP)

    WB analysis of mouse lymph using 17458-1-AP

    mouse lymph tissue were subjected to SDS PAGE followed by western blot with 17458-1-AP (RTKN2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RTKN2 Polyclonal antibody (17458-1-AP)

    WB analysis of HEK-293 using 17458-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17458-1-AP (RTKN2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using RTKN2 Polyclonal antibody (17458-1-AP)

    WB analysis of Raji using 17458-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 17458-1-AP (RTKN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using RTKN2 Polyclonal antibody (17458-1-AP)

    IHC staining of human testis using 17458-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using RTKN2 Polyclonal antibody (17458-1-AP)

    IHC staining of human testis using 17458-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using RTKN2 Polyclonal antibody (17458-1-AP)

    IHC staining of human skin using 17458-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using RTKN2 Polyclonal antibody (17458-1-AP)

    IHC staining of human skin using 17458-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using RTKN2 Polyclonal antibody (17458-1-AP)

    IHC staining of human brain using 17458-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using RTKN2 Polyclonal antibody (17458-1-AP)

    IHC staining of human brain using 17458-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using RTKN2 Polyclonal antibody (17458-1-AP)

    IHC staining of human placenta using 17458-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using RTKN2 Polyclonal antibody (17458-1-AP)

    IHC staining of human placenta using 17458-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using RTKN2 Polyclonal antibody (17458-1-AP)

    IF Staining of HEK-293 using 17458-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHL-60 cells, mouse lymph tissue, Raji cells, HEK-293 cells
Positive IHC detected inhuman testis tissue, human brain tissue, human placenta tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17458-1-AP targets RTKN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RTKN2 fusion protein Ag11098
Full Name rhotekin 2
Calculated Molecular Weight163aa,19 kDa; 609aa,69 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC025765
Gene Symbol RTKN2
Gene ID (NCBI) 219790
RRIDAB_2034939
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RTKN2 antibody 17458-1-APDownload protocol
IHC protocol for RTKN2 antibody 17458-1-APDownload protocol
IF protocol for RTKN2 antibody 17458-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Rep

Cellular hormetic response to 27-hydroxycholesterol promotes neuroprotection through AICD induction of MAST4 abundance and kinase activity.

Authors - Brendan Gongol
  • KD Validated
View Article
humanWB,IHC

J Viral Hepat

Depletion of circ_0088046 suppressed cell growth and motility of hepatocellular carcinoma via circ_0088046-miR-1299-RTKN2 ceRNA pathway

Authors - Li Zhang
View Article
humanWB

Sci Rep

RTKN2 knockdown alleviates the malignancy of breast cancer cells by regulating the Wnt/β-catenin pathway

Authors - Xiaomei Zhang
View Article