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- KD/KO Validated
RRAGB Polyclonal antibody
RRAGB Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IP, IF, RIP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 13023-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | human testis tissue, human brain tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
| RIP | See 1 publications below |
Product Information
13023-1-AP targets RRAGB in WB, IP, IF, RIP, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RRAGB fusion protein Ag4116 |
| Full Name | Ras-related GTP binding B |
| Calculated Molecular Weight | 346 aa, 40 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC034726 |
| Gene Symbol | RRAGB |
| Gene ID (NCBI) | 10325 |
| RRID | AB_2180064 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RRAGB antibody 13023-1-AP | Download protocol |
| IHC protocol for RRAGB antibody 13023-1-AP | Download protocol |
| IP protocol for RRAGB antibody 13023-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cancer circEXOC6B interacting with RRAGB, an mTORC1 activator, inhibits the progression of colorectal cancer by antagonizing the HIF1A-RRAGB-mTORC1 positive feedback loop. | ||
J Clin Invest YAP plays a crucial role in the development of cardiomyopathy in lysosomal storage diseases.
| ||
PLoS One Ursolic Acid Inhibits Leucine-Stimulated mTORC1 Signaling by Suppressing mTOR Localization to Lysosome. | ||
Physiol Rep Tissue-specific expression differences in Ras-related GTP-binding proteins in male rats | ||
Biochem Biophys Res Commun RRAGB-mediated suppression of PI3K/AKT exerts anti-cancer role in glioblastoma | ||
Cell Death Dis Inosine enhances tumor mitochondrial respiration by inducing Rag GTPases and nascent protein synthesis under nutrient starvation |
