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- KD/KO Validated
RPS26 Polyclonal antibody
RPS26 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA, PLA
Conjugate
Unconjugated
Cat no : 14909-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse ovary tissue, mouse lung tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human breast cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 11 publications below |
| IHC | See 1 publications below |
| IP | See 1 publications below |
Product Information
14909-1-AP targets RPS26 in WB, IHC, IF/ICC, IP, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RPS26 fusion protein Ag6706 |
| Full Name | ribosomal protein S26 |
| Calculated Molecular Weight | 13 kDa |
| Observed Molecular Weight | 18-21 kDa |
| GenBank Accession Number | BC002604 |
| Gene Symbol | RPS26 |
| Gene ID (NCBI) | 6231 |
| RRID | AB_2180361 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Diamond-Blackfan anemia (DBA) is a congenital red cell aplasia that is usually diagnosed during early infancy, and strikingly, in addition to defects in red cell maturation the disorder is associated with various physical anomalies in about 40% of patients. To date, heterozygous mutations in nine RP genes have been conclusively associated with DBA, and RPS26 is one of them. There are nine mutation found in RPS26 from DBA patients.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RPS26 antibody 14909-1-AP | Download protocol |
| IHC protocol for RPS26 antibody 14909-1-AP | Download protocol |
| IF protocol for RPS26 antibody 14909-1-AP | Download protocol |
| IP protocol for RPS26 antibody 14909-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages | ||
Nat Commun Genome-wide analyses of non-syndromic cleft lip with palate identify 14 novel loci and genetic heterogeneity. | ||
Elife Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons. | ||
Oncogene The ribosomal protein S26 regulates p53 activity in response to DNA damage.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Shinford (Verified Customer) (09-24-2023) | The esophageal cancer cell line is used. The antibody works very stably, and the concentration can be from 1:1000 to 1:2000.
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