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RPS13 Polyclonal antibody

RPS13 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16680-1-AP

Synonyms

Small ribosomal subunit protein uS15, ribosomal protein S13, 40S ribosomal protein S13

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPS13 Polyclonal antibody (16680-1-AP)

    WB analysis using 16680-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16680-1-AP (RPS13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RPS13 Polyclonal antibody (16680-1-AP)

    IHC staining of human colon cancer using 16680-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16680-1-AP (RPS13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RPS13 Polyclonal antibody (16680-1-AP)

    IHC staining of human colon cancer using 16680-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16680-1-AP (RPS13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RPS13 Polyclonal antibody (16680-1-AP)

    IF Staining of HeLa using 16680-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 16680-1-AP (RPS13 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inMCF-7 cells, mouse oterus tissue, rat oterus tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

16680-1-AP targets RPS13 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPS13 fusion protein Ag10008
Full Name ribosomal protein S13
Calculated Molecular Weight 17 kDa
Observed Molecular Weight 14 kDa, 17 kDa
GenBank Accession NumberBC006772
Gene Symbol RPS13
Gene ID (NCBI) 6207
RRIDAB_2182500
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPS13, also named as 40S ribosomal protein S13, belongs to the ribosomal protein S15P family. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. RPS13 is located in the cytoplasm. It is co-transcribed with two U14 small nucleolar RNA genes, which are located in its third and fifth introns. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Protocols

Product Specific Protocols
WB protocol for RPS13 antibody 16680-1-APDownload protocol
IHC protocol for RPS13 antibody 16680-1-APDownload protocol
IF protocol for RPS13 antibody 16680-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Cell

Circadian clocks are modulated by compartmentalized oscillating translation

Authors - Yanrong Zhuang
View Article
humanWB

Stem Cells

Genome-wide studies reveal that Lin28 enhances the translation of genes important for growth and survival of human embryonic stem cells.

Authors - Peng Shuping S
View Article
humanWB

N/A

N/A

Authors - N/A
View Article
WB

Cell

An E3 ligase network engages GCN1 to promote the degradation of translation factors on stalled ribosomes

Authors - Keely Oltion
View Article