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RPL32 Polyclonal antibody

RPL32 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16629-1-AP

Synonyms

60S ribosomal protein L32, PP9932, ribosomal protein L32, RPL32

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RPL32 Polyclonal antibody (16629-1-AP)

    WB analysis of HeLa using 16629-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16629-1-AP (RPL32 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using RPL32 Polyclonal antibody (16629-1-AP)

    WB analysis of A549 using 16629-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 16629-1-AP (RPL32 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RPL32 Polyclonal antibody (16629-1-AP)

    WB analysis of HeLa using 16629-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16629-1-AP (RPL32 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using RPL32 Polyclonal antibody (16629-1-AP)

    WB analysis of mouse lung using 16629-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 16629-1-AP (RPL32 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RPL32 Polyclonal antibody (16629-1-AP)

    WB analysis of HepG2 using 16629-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16629-1-AP (RPL32 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, mouse lung tissue, HepG2 cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16629-1-AP targets RPL32 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL32 fusion protein Ag9960
Full Name ribosomal protein L32
Calculated Molecular Weight 135 aa, 16 kDa
Observed Molecular Weight 16-18 kDa
GenBank Accession NumberBC070209
Gene Symbol RPL32
Gene ID (NCBI) 6161
RRIDAB_2254034
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RPL32 antibody 16629-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols