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  • Featured Product
  • KD/KO Validated

RPL31 Polyclonal antibody

RPL31 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16497-1-AP

Synonyms

ribosomal protein L31, Large ribosomal subunit protein eL31, 60S ribosomal protein L31

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using RPL31 Polyclonal antibody (16497-1-AP)

    WB analysis of HepG2 using 16497-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16497-1-AP (RPL31 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RPL31 Polyclonal antibody (16497-1-AP)

    WB analysis of HepG2 using 16497-1-AP

    WB result of RPL31 antibody (16497-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPL31 transfected HepG2 cells.

  • Western Blot (WB) analysis of NIH/3T3 cells using RPL31 Polyclonal antibody (16497-1-AP)

    WB analysis of NIH/3T3 using 16497-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16497-1-AP (RPL31 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using RPL31 Polyclonal antibody (16497-1-AP)

    WB analysis of NIH/3T3 using 16497-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16497-1-AP (RPL31 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using RPL31 Polyclonal antibody (16497-1-AP)

    WB analysis of mouse liver using 16497-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16497-1-AP (RPL31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using RPL31 Polyclonal antibody (16497-1-AP)

    WB analysis of mouse liver using 16497-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16497-1-AP (RPL31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPL31 Polyclonal antibody (16497-1-AP)

    IF Staining of HepG2 using 16497-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 16497-1-AP (RPL31 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, mouse liver tissue, NIH/3T3 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16497-1-AP targets RPL31 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL31 fusion protein Ag9648
Full Name ribosomal protein L31
Calculated Molecular Weight 125 aa, 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC017343
Gene Symbol RPL31
Gene ID (NCBI) 6160
RRIDAB_2181772
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. Ribosomal protein L31 gene is a component of the 60S large ribosomal subunit encoded by RPL31 gene, while ribosomal protein L31 (RPL31) is an important constituent of peptidyltransferase center [PMID:22714919].

Protocols

Product Specific Protocols
WB protocol for RPL31 antibody 16497-1-APDownload protocol
IF protocol for RPL31 antibody 16497-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Curr Biol

RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.

Authors - Long Jiang
View Article
mouseIF

J Med Genet

Bi-allelic variants in chromatoid body protein TDRD6 cause spermiogenesis defects and severe oligoasthenoteratozoospermia in humans

Authors - Rui Guo
View Article
humanWB

iScience

TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment

Authors - Magdalena Wolczyk
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Wei (Verified Customer) (01-30-2020)

Weak band around 15kDa

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse Heart