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RPL24 Polyclonal antibody
RPL24 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IF/ICC, IP, RIP, ELISA
Conjugate
Unconjugated
Cat no : 17082-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HEK-293 cells, Jurkat cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human placenta tissue, human kidney tissue, human liver tissue, human spleen tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 11 publications below |
| IHC | See 3 publications below |
| IF | See 4 publications below |
| IP | See 1 publications below |
| RIP | See 1 publications below |
Product Information
17082-1-AP targets RPL24 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RPL24 fusion protein Ag7085 |
| Full Name | ribosomal protein L24 |
| Calculated Molecular Weight | 18 kDa |
| Observed Molecular Weight | 21-23 kDa |
| GenBank Accession Number | BC000690 |
| Gene Symbol | RPL24 |
| Gene ID (NCBI) | 6152 |
| RRID | AB_2181728 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. RPL24 is one component of the large (60S) subunits that promote the translation of uORF-containing mRNAsgene The mutation in Rpl24 result in impairment of mRNA splicing and L24 production, which in turn affects ribosome biogenesis, protein synthesis and the cell cycle. PMID:20799971]. Also RPL24 (ribosomal protein L24) is a key factor for translation reinitiation of downstream ORFs on the polycistronic cauliflower mosaic virus 35S RNA transcription unit, and may have a role in gynoecuim development.[PMID:15270688]
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RPL24 antibody 17082-1-AP | Download protocol |
| IHC protocol for RPL24 antibody 17082-1-AP | Download protocol |
| IF protocol for RPL24 antibody 17082-1-AP | Download protocol |
| IP protocol for RPL24 antibody 17082-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Ribosome ADP-ribosylation inhibits translation and maintains proteostasis in cancers.
| ||
Cell Stem Cell HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis. | ||
Dev Cell Axonal endoplasmic reticulum tubules control local translation via P180/RRBP1-mediated ribosome interactions | ||
Cell Mol Life Sci Ribosomal protein L24 mediates mammalian microRNA processing in an evolutionarily conserved manner |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Erica (Verified Customer) (05-15-2019) | The antibody works very well for WB in both Human HEK and MEF cells. I used 1:2000 dilution in 2%BSA, and it always work well. It doesn't work very well for IP though..
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