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RPL18A Polyclonal antibody

RPL18A Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15751-1-AP

Synonyms

60S ribosomal protein L18a, ribosomal protein L18a, RPL18A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPL18A Polyclonal antibody (15751-1-AP)

    WB analysis using 15751-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15751-1-AP (RPL18A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RPL18A Polyclonal antibody (15751-1-AP)

    WB analysis of HeLa using 15751-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15751-1-AP (RPL18A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using RPL18A Polyclonal antibody (15751-1-AP)

    IP experiment of Jurkat using 15751-1-AP

    IP result of anti-RPL18A (IP:15751-1-AP, 4ug; Detection:15751-1-AP 1:500) with Jurkat cells lysate 3200 ug.

  • Immunohistochemistry (IHC) staining of human brain tissue using RPL18A Polyclonal antibody (15751-1-AP)

    IHC staining of human brain using 15751-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15751-1-AP (RPL18A antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using RPL18A Polyclonal antibody (15751-1-AP)

    IHC staining of human brain using 15751-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15751-1-AP (RPL18A antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15751-1-AP targets RPL18A in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL18A fusion protein Ag8251
Full Name ribosomal protein L18a
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC007512
Gene Symbol RPL18A
Gene ID (NCBI) 6142
RRIDAB_2181574
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RPL18A antibody 15751-1-APDownload protocol
IHC protocol for RPL18A antibody 15751-1-APDownload protocol
IP protocol for RPL18A antibody 15751-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols