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RPL18A Polyclonal antibody

RPL18A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14653-1-AP

Synonyms

ribosomal protein L18a, Large ribosomal subunit protein eL20, 60S ribosomal protein L18a

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using RPL18A Polyclonal antibody (14653-1-AP)

    WB analysis of HepG2 using 14653-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RPL18A Polyclonal antibody (14653-1-AP)

    WB analysis of HeLa using 14653-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using RPL18A Polyclonal antibody (14653-1-AP)

    WB analysis of human brain using 14653-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using RPL18A Polyclonal antibody (14653-1-AP)

    WB analysis of mouse lung using 14653-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using RPL18A Polyclonal antibody (14653-1-AP)

    WB analysis of PC-3 using 14653-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RPL18A Polyclonal antibody (14653-1-AP)

    IHC staining of human prostate cancer using 14653-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14653-1-AP (RPL18A antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RPL18A Polyclonal antibody (14653-1-AP)

    IHC staining of human prostate cancer using 14653-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14653-1-AP (RPL18A antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPL18A Polyclonal antibody (14653-1-AP)

    IF Staining of HepG2 using 14653-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL18A antibody (14653-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPL18A Polyclonal antibody (14653-1-AP)

    IF Staining of HepG2 using 14653-1-AP

    Immunofluorescent analysis of HepG2 cells, using RPL18A antibody 14653-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, human brain tissue, mouse lung tissue, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

14653-1-AP targets RPL18A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL18A fusion protein Ag6251
Full Name ribosomal protein L18a
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC066319
Gene Symbol RPL18A
Gene ID (NCBI) 6142
RRIDAB_2181576
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPL18A, also named as 60S ribosomal protein L18a(Ribosomal Protein L18a), located on 19p13.11, is a protein coding gene. RPL18A is a member of the L18AE family of ribosomal proteins that is a component of the 60S subunit. The encoded protein may play a role in viral replication by interacting with the hepatitis C virus internal ribosome entry site (IRES). Diseases such as Hepatitis C Virus and Omsk Hemorrhagic Fever are associated with RPL18A. The molecular weight of RPL18A is 23 kDa.

Protocols

Product Specific Protocols
WB protocol for RPL18A antibody 14653-1-APDownload protocol
IHC protocol for RPL18A antibody 14653-1-APDownload protocol
IF protocol for RPL18A antibody 14653-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Rep

Down-regulation of HSP60 Suppresses the Proliferation of Glioblastoma Cells via the ROS/AMPK/mTOR Pathway.

Authors - Haiping Tang
View Article
mouseWB

Genetics

Quantitative changes in gimap3 and gimap5 expression modify mitochondrial DNA segregation in mice.

Authors - Riikka Jokinen
View Article
mouseWB

J Pineal Res

Amelioration of gamma irradiation-induced salivary gland damage in mice using melatonin

Authors - Jingming Ren
View Article
ratWB

EMBO Rep

Molecular view of ER membrane remodeling by the Sec61/TRAP translocon

Authors - Sudeep Karki
View Article