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RPL15 Polyclonal antibody

RPL15 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16740-1-AP

Synonyms

RPLY10, RPL10, ribosomal protein L15, Large ribosomal subunit protein eL15, EC45

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using RPL15 Polyclonal antibody (16740-1-AP)

    WB analysis of COLO 320 using 16740-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 16740-1-AP (RPL15 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using RPL15 Polyclonal antibody (16740-1-AP)

    WB analysis of K-562 using 16740-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 16740-1-AP (RPL15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RPL15 Polyclonal antibody (16740-1-AP)

    WB analysis of HeLa using 16740-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16740-1-AP (RPL15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using RPL15 Polyclonal antibody (16740-1-AP)

    WB analysis of mouse brain using 16740-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16740-1-AP (RPL15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SGC-7901 cells using RPL15 Polyclonal antibody (16740-1-AP)

    WB analysis of SGC-7901 using 16740-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 16740-1-AP (RPL15 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using RPL15 Polyclonal antibody (16740-1-AP)

    IHC staining of human colon using 16740-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16740-1-AP (RPL15 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RPL15 Polyclonal antibody (16740-1-AP)

    IF Staining of HeLa using 16740-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16740-1-AP (RPL15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inCOLO 320 cells, HeLa cells, K-562 cells, mouse brain tissue, SGC-7901 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16740-1-AP targets RPL15 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL15 fusion protein Ag9948
Full Name ribosomal protein L15
Calculated Molecular Weight 204 aa, 24 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC070328
Gene Symbol RPL15
Gene ID (NCBI) 6138
RRIDAB_2301193
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RPL15 antibody 16740-1-APDownload protocol
IHC protocol for RPL15 antibody 16740-1-APDownload protocol
IF protocol for RPL15 antibody 16740-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Dev Cell

Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.

Authors - Yuqing Lei
View Article
pigWB

Front Immunol

Foot-and-Mouth Disease Virus Counteracts on Internal Ribosome Entry Site Suppression by G3BP1 and Inhibits G3BP1-Mediated Stress Granule Assembly via Post-Translational Mechanisms.

Authors - Xu Ye
View Article
humanWB

Cell

Human hematopoietic stem cell vulnerability to ferroptosis

Authors - Jiawei Zhao
View Article
humanIF

Cell Rep

Senescent cells form nuclear foci that contain the 26S proteasome

Authors - Tomohiro Iriki
View Article
humanWB

Cell Rep

ZNF692 organizes a hub specialized in 40S ribosomal subunit maturation enhancing translation in rapidly proliferating cells

Authors - M Carmen Lafita-Navarro
View Article