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  • Featured Product
  • KD/KO Validated

RPE Polyclonal antibody

RPE Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12168-2-AP

Synonyms

Ribulose-phosphate 3-epimerase, Ribulose-5-phosphate-3-epimerase, HUSSY-17, EC:5.1.3.1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293T cells using RPE Polyclonal antibody (12168-2-AP)

    WB analysis of HEK-293T using 12168-2-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 12168-2-AP (RPE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using RPE Polyclonal antibody (12168-2-AP)

    WB analysis of mouse thymus using 12168-2-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12168-2-AP (RPE antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RPE Polyclonal antibody (12168-2-AP)

    WB analysis of HeLa using 12168-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12168-2-AP (RPE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using RPE Polyclonal antibody (12168-2-AP)

    WB analysis of K-562 using 12168-2-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12168-2-AP (RPE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using RPE Polyclonal antibody (12168-2-AP)

    IF Staining of RAW 264.7 using 12168-2-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using RPE antibody (12168-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293T cells, mouse thymus tissue, HeLa cells, K-562 cells
Positive IF/ICC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12168-2-AP targets RPE in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPE fusion protein Ag2810
Full Name ribulose-5-phosphate-3-epimerase
Calculated Molecular Weight 228 aa, 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC016764
Gene Symbol RPE
Gene ID (NCBI) 6120
RRIDAB_2253939
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RPE antibody 12168-2-APDownload protocol
IF protocol for RPE antibody 12168-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

mTOR Overcomes Multiple Metabolic Restrictions to Enable HIV-1 Reverse Transcription and Intracellular Transport.

Authors - Harry E Taylor
View Article
humanWB

Nat Metab

Salvage of ribose from uridine or RNA supports glycolysis in nutrient-limited conditions

Authors - Owen S Skinner
  • KO Validated
View Article