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DPCD Polyclonal antibody

DPCD Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20936-1-AP

Synonyms

DPCD, Protein DPCD, RP11 529I10.4, RP11-529I10.4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DPCD Polyclonal antibody (20936-1-AP)

    WB analysis using 20936-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20936-1-AP (DPCD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using DPCD Polyclonal antibody (20936-1-AP)

    WB analysis of human testis using 20936-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 20936-1-AP (DPCD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DPCD Polyclonal antibody (20936-1-AP)

    IHC staining of mouse testis using 20936-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20936-1-AP (DPCD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DPCD Polyclonal antibody (20936-1-AP)

    IHC staining of mouse testis using 20936-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20936-1-AP (DPCD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using DPCD Polyclonal antibody (20936-1-AP)

    IHC staining of human heart using 20936-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart slide using 20936-1-AP (DPCD antibody at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human heart tissue using DPCD Polyclonal antibody (20936-1-AP)

    IHC staining of human heart using 20936-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart slide using 20936-1-AP (DPCD antibody at dilution of 1:50.

Tested Applications

Positive WB detected inmouse testis tissue, human testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20936-1-AP is DPCD Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DPCD fusion protein Ag15029
Full Name deleted in a mouse model of primary ciliary dyskinesia
Calculated Molecular Weight 203 aa, 23 kDa
Observed Molecular Weight 23-26 kDa
GenBank Accession NumberBC031695
Gene Symbol DPCD
Gene ID (NCBI) 25911
RRIDAB_10734461
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DPCD (for deleted in a mouse model of primary ciliary dyskinesia), is predicted to code for a protein of 23 kDa which remains a novel candidate gene for PCD (Primary ciliary dyskinesia).

Protocols

Product Specific Protocols
WB protocol for DPCD antibody 20936-1-APDownload protocol
IHC protocol for DPCD antibody 20936-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Cell Rep

DPCD is a regulator of R2TP in ciliogenesis initiation through Akt signaling

Authors - Yu-Qian Mao
View Article
humanWB,IF

Sci Rep

TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins.

Authors - S Prpar Mihevc
View Article