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ROPN1L Polyclonal antibody

ROPN1L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12565-1-AP

Synonyms

ropporin 1 like, ROPN1-like protein, ROPN1 like protein, ASP, AKAP-associated sperm protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ROPN1L Polyclonal antibody (12565-1-AP)

    WB analysis using 12565-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12565-1-AP (ROPN1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ROPN1L Polyclonal antibody (12565-1-AP)

    IHC staining of mouse testis using 12565-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12565-1-AP (ROPN1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ROPN1L Polyclonal antibody (12565-1-AP)

    IHC staining of mouse testis using 12565-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12565-1-AP (ROPN1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ROPN1L Polyclonal antibody (12565-1-AP)

    IHC staining of human colon cancer using 12565-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12565-1-AP (ROPN1L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ROPN1L Polyclonal antibody (12565-1-AP)

    IHC staining of human colon cancer using 12565-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12565-1-AP (ROPN1L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12565-1-AP targets ROPN1L in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ROPN1L fusion protein Ag3178
Full Name ropporin 1-like
Calculated Molecular Weight 230 aa, 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC014607
Gene Symbol ROPN1L
Gene ID (NCBI) 83853
RRIDAB_10638785
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ROPN1L (Rhophilin Associated Tail Protein 1 Like) is a protein-coding gene that is a member of the ropporin family. ROPN1L plays a role in sperm function, as mutations in ROPN1L can cause defects in the fibrous sheath integrity, sperm motility, and PKA-dependent signaling processes, leading to male infertility.

Protocols

Product Specific Protocols
WB protocol for ROPN1L antibody 12565-1-APDownload protocol
IHC protocol for ROPN1L antibody 12565-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols