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  • Featured Product
  • KD/KO Validated

RNMTL1 Polyclonal antibody

RNMTL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14707-1-AP

Synonyms

RMTL1, MRM3, HC90, EC:2.1.1.-, 16S rRNA [Gm1370] 2'-O-methyltransferase

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RNMTL1 Polyclonal antibody (14707-1-AP)

    WB analysis of HeLa using 14707-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14707-1-AP (RNMTL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RNMTL1 Polyclonal antibody (14707-1-AP)

    WB analysis of HEK-293 using 14707-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14707-1-AP (RNMTL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using RNMTL1 Polyclonal antibody (14707-1-AP)

    IHC staining of human liver using 14707-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14707-1-AP (RNMTL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using RNMTL1 Polyclonal antibody (14707-1-AP)

    IHC staining of human liver using 14707-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14707-1-AP (RNMTL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RNMTL1 Polyclonal antibody (14707-1-AP)

    IF Staining of HeLa using 14707-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RNMTL1 antibody (14707-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14707-1-AP targets RNMTL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNMTL1 fusion protein Ag6399
Full Name RNA methyltransferase like 1
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC050614
Gene Symbol RNMTL1
Gene ID (NCBI) 55178
RRIDAB_2181563
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The RNA methyltransferase like 1 gene (RNMTL1) is one of thirteen newly discovered genes within a 116 Kb segment of the chromosome 17p13.3, which suffers from a high frequent loss of heterozygosity in human hepatocellular carcinoma. Its amino acid sequence was identified a high conserved region with methylases from various microorganisms, suggesting to have function to transfer methyl group to rRNA molecule [PMID:12296377].

Protocols

Product Specific Protocols
WB protocol for RNMTL1 antibody 14707-1-APDownload protocol
IHC protocol for RNMTL1 antibody 14707-1-APDownload protocol
IF protocol for RNMTL1 antibody 14707-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human, mouseWB,IF

J Biol Chem

Mitochondrial rRNA Methyltransferase Family Members are Positioned to Modify Nascent rRNA in Foci Near the mtDNA Nucleoid.

Authors - Lee Ken-Wing KW
  • KD Validated
View Article