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  • Featured Product
  • KD/KO Validated

RNF34 Polyclonal antibody

RNF34 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10629-1-AP

Synonyms

CARP 1, Caspase regulator CARP1, FYVE RING finger protein Momo, hRFI, RFI, RIF, RIFF, ring finger protein 34, RING finger protein RIFF, RNF34

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RNF34 Polyclonal antibody (10629-1-AP)

    IHC staining of human colon cancer using 10629-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10629-1-AP (RNF34 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RNF34 Polyclonal antibody (10629-1-AP)

    IHC staining of human colon cancer using 10629-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10629-1-AP (RNF34 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RNF34 Polyclonal antibody (10629-1-AP)

    IHC staining of human breast cancer using 10629-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10629-1-AP (RNF34 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RNF34 Polyclonal antibody (10629-1-AP)

    IHC staining of human breast cancer using 10629-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10629-1-AP (RNF34 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10629-1-AP targets RNF34 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF34 fusion protein Ag0982
Full Name ring finger protein 34
Calculated Molecular Weight 42 kDa
GenBank Accession NumberBC007826
Gene Symbol RNF34
Gene ID (NCBI) 80196
RRIDAB_2301135
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNF34 is an E3 ubiquitin ligase that regulates protein substrates by post-translational modification. It is associated with regulating postsynaptic γ2-GABAAR clustering and GABAergic synaptic innervation in hippocampal neurons, indicating the importance of RNF34 in regulating neurological function.It is preferentially expressed in esophageal, gastric, and colorectal cancers, suggesting a possible association between RNF34 and the development of digestive tract cancers. Immunoblots showed a single 42-kDa protein band corresponding to the molecular weight of RNF34.(PMID: 31704983, PMID: 25193658)

Protocols

Product Specific Protocols
IHC protocol for RNF34 antibody 10629-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

Nat Commun

A ZFYVE21-Rubicon-RNF34 signaling complex promotes endosome-associated inflammasome activity in endothelial cells

Authors - Xue Li
  • KD Validated
View Article