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RNF168 Polyclonal antibody
RNF168 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 21393-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, HeLa cells, human brain tissue, Jurkat cells, Transfected HEK-293 cells |
Positive IP detected in | HepG2 cells |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 7 publications below |
IF | See 3 publications below |
IP | See 3 publications below |
CoIP | See 1 publications below |
Product Information
21393-1-AP targets RNF168 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | RNF168 fusion protein Ag16055 |
Full Name | ring finger protein 168 |
Calculated Molecular Weight | 571 aa, 65 kDa |
Observed Molecular Weight | 65-70 kDa |
GenBank Accession Number | BC033791 |
Gene Symbol | RNF168 |
Gene ID (NCBI) | 165918 |
RRID | AB_10733883 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for RNF168 antibody 21393-1-AP | Download protocol |
IF protocol for RNF168 antibody 21393-1-AP | Download protocol |
IP protocol for RNF168 antibody 21393-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Cell A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis | ||
Nat Commun Non-canonical function of DGCR8 in DNA double-strand break repair signaling and tumor radioresistance. | ||
Nucleic Acids Res GLP-catalyzed H4K16me1 promotes 53BP1 recruitment to permit DNA damage repair and cell survival. | ||
Theranostics A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis. | ||
J Cell Biol DUX4-induced HSATII transcription causes KDM2A/B-PRC1 nuclear foci and impairs DNA damage response | ||
EMBO Rep An LTR retrotransposon-derived lncRNA interacts with RNF169 to promote homologous recombination. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Shunbin (Verified Customer) (05-14-2024) | Great. A single specific band, no background
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FH Shunbin (Verified Customer) (05-14-2024) | Great. A single specific band without background
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