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RNF144B/IBRDC2 Polyclonal antibody

RNF144B/IBRDC2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 26306-1-AP

Synonyms

IBRDC2, KIAA0161, p53RFP, ring finger protein 144B, RNF144B

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RNF144B/IBRDC2 Polyclonal antibody (26306-1-AP)

    WB analysis of HeLa using 26306-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26306-1-AP (RNF144B/IBRDC2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using RNF144B/IBRDC2 Polyclonal antibody (26306-1-AP)

    IP experiment of HeLa using 26306-1-AP

    IP result of anti-RNF144B/IBRDC2 (IP:26306-1-AP, 4ug; Detection:26306-1-AP 1:300) with HeLa cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using RNF144B/IBRDC2 Polyclonal antibody (26306-1-AP)

    IHC staining of human kidney using 26306-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26306-1-AP (RNF144B/IBRDC2 antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using RNF144B/IBRDC2 Polyclonal antibody (26306-1-AP)

    IHC staining of human kidney using 26306-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26306-1-AP (RNF144B/IBRDC2 antibody at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26306-1-AP is RNF144B/IBRDC2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF144B/IBRDC2 fusion protein Ag23679
Full Name ring finger protein 144B
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC063311
Gene Symbol RNF144B
Gene ID (NCBI) 255488
RRIDAB_2880473
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RNF144B/IBRDC2 antibody 26306-1-APDownload protocol
IHC protocol for RNF144B/IBRDC2 antibody 26306-1-APDownload protocol
IP protocol for RNF144B/IBRDC2 antibody 26306-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP,CoIP

EMBO Rep

RNF144B negatively regulates antiviral immunity by targeting MDA5 for autophagic degradation

Authors - Guoxiu Li
View Article