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  • KD/KO Validated

RND3 Monoclonal antibody

RND3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3H8F9

Cat no : 66228-1-Ig

Synonyms

ARHE, memB, Protein MemB, Rho family GTPase 3, Rho8, RhoE, RND3



Tested Applications

Positive WB detected infetal human brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66228-1-Ig targets RND3 in WB, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RND3 fusion protein Ag18839
Full Name Rho family GTPase 3
Calculated Molecular Weight 244 aa, 27 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC012513
Gene Symbol RND3
Gene ID (NCBI) 390
RRIDAB_2881619
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rnd3, also known as RhoE, belongs to the Rnd subclass of the Rho family of small guanosine triphosphate (GTP)-binding proteins. Rnd proteins are unique due to their inability to switch from a GTP-bound to GDP-bound conformation. The basic role of Rnd3 is to report as an endogenous antagonist of RhoA signaling-mediated actin cytoskeleton dynamics, which specifically contributes to cell migration and neuron polarity. Rnd3 also plays a critical role in arresting cell cycle distribution, inhibiting cell growth, and inducing apoptosis and differentiation.

Protocols

Product Specific Protocols
WB protocol for RND3 antibody 66228-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Ther Nucleic Acids

Downregulated lncRNA HOXA11-AS Affects Trophoblast Cell Proliferation and Migration by Regulating RND3 and HOXA7 Expression in PE.

Authors - Yetao Xu
humanWB

Int J Mol Med

Ingenuity pathway analysis of differentially expressed genes involved in signaling pathways and molecular networks in RhoE gene‑edited cardiomyocytes.

Authors - Zhongming Shao
  • KO Validated
humanWB

Virology

Transcriptomic analysis reveals crucial regulatory roles of immediate-early response genes and related signaling pathways in coronavirus infectious bronchitis virus infection

Authors - Li Xia Yuan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Yuan (Verified Customer) (12-10-2020)

This antibody is very good quality and can detect RND3 in both U937 and PBMC cells.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: U937 & PBMC