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RND3 Monoclonal antibody
RND3 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, pig
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
3H8F9
Cat no : 66228-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | fetal human brain tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 3 publications below |
Product Information
66228-1-Ig targets RND3 in WB, ELISA applications and shows reactivity with human, pig samples.
Tested Reactivity | human, pig |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | RND3 fusion protein Ag18839 |
Full Name | Rho family GTPase 3 |
Calculated Molecular Weight | 244 aa, 27 kDa |
Observed Molecular Weight | 29 kDa |
GenBank Accession Number | BC012513 |
Gene Symbol | RND3 |
Gene ID (NCBI) | 390 |
RRID | AB_2881619 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Rnd3, also known as RhoE, belongs to the Rnd subclass of the Rho family of small guanosine triphosphate (GTP)-binding proteins. Rnd proteins are unique due to their inability to switch from a GTP-bound to GDP-bound conformation. The basic role of Rnd3 is to report as an endogenous antagonist of RhoA signaling-mediated actin cytoskeleton dynamics, which specifically contributes to cell migration and neuron polarity. Rnd3 also plays a critical role in arresting cell cycle distribution, inhibiting cell growth, and inducing apoptosis and differentiation.
Protocols
Product Specific Protocols | |
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WB protocol for RND3 antibody 66228-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Ther Nucleic Acids Downregulated lncRNA HOXA11-AS Affects Trophoblast Cell Proliferation and Migration by Regulating RND3 and HOXA7 Expression in PE. | ||
Int J Mol Med Ingenuity pathway analysis of differentially expressed genes involved in signaling pathways and molecular networks in RhoE gene‑edited cardiomyocytes.
| ||
Virology Transcriptomic analysis reveals crucial regulatory roles of immediate-early response genes and related signaling pathways in coronavirus infectious bronchitis virus infection |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Yuan (Verified Customer) (12-10-2020) | This antibody is very good quality and can detect RND3 in both U937 and PBMC cells.
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