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  • Featured Product
  • KD/KO Validated

RNASEH2C Polyclonal antibody

RNASEH2C Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16518-1-AP

Synonyms

AGS3, AYP1, Ribonuclease H2 subunit C, ribonuclease H2, subunit C, Ribonuclease HI subunit C, RNase H1 small subunit, RNase H2 subunit C, RNASEH2C

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNASEH2C Polyclonal antibody (16518-1-AP)

    WB analysis using 16518-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16518-1-AP (RNASEH2C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using RNASEH2C Polyclonal antibody (16518-1-AP)

    WB analysis of human testis using 16518-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 16518-1-AP (RNASEH2C antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RNASEH2C Polyclonal antibody (16518-1-AP)

    WB analysis of HEK-293 using 16518-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16518-1-AP (RNASEH2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human spleen tissue using RNASEH2C Polyclonal antibody (16518-1-AP)

    WB analysis of human spleen using 16518-1-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 16518-1-AP (RNASEH2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using RNASEH2C Polyclonal antibody (16518-1-AP)

    IP experiment of HEK-293 using 16518-1-AP

    IP result of anti-RNASEH2C (IP:16518-1-AP, 3ug; Detection:16518-1-AP 1:500) with HEK-293 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human brain tissue using RNASEH2C Polyclonal antibody (16518-1-AP)

    IHC staining of human brain using 16518-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using RNASEH2C Polyclonal antibody (16518-1-AP)

    IHC staining of human brain using 16518-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using RNASEH2C Polyclonal antibody (16518-1-AP)

    IHC staining of human spleen using 16518-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using RNASEH2C Polyclonal antibody (16518-1-AP)

    IHC staining of human spleen using 16518-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using RNASEH2C Polyclonal antibody (16518-1-AP)

    IHC staining of human lung using 16518-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using RNASEH2C Polyclonal antibody (16518-1-AP)

    IHC staining of human lung using 16518-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using RNASEH2C Polyclonal antibody (16518-1-AP)

    IHC staining of human skin using 16518-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using RNASEH2C Polyclonal antibody (16518-1-AP)

    IHC staining of human skin using 16518-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, human spleen tissue, human testis tissue, HeLa cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman brain tissue, human lung tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16518-1-AP targets RNASEH2C in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNASEH2C fusion protein Ag9739
Full Name ribonuclease H2, subunit C
Calculated Molecular Weight 164 aa, 18 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC023588
Gene Symbol RNASEH2C
Gene ID (NCBI) 84153
RRIDAB_2181648
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RNASEH2C antibody 16518-1-APDownload protocol
IHC protocol for RNASEH2C antibody 16518-1-APDownload protocol
IP protocol for RNASEH2C antibody 16518-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions.

Authors - Michal Zimmermann
View Article
humanWB

Hum Mol Genet

Reduction of hRNase H2 activity in Aicardi-Goutières Syndrome cells leads to replication stress and genome instability.

Authors - Sara Pizzi
View Article
humanWB

J Biol Chem

Analysis of ribonucleotide removal from DNA by human nucleotide excision repair.

Authors - Laura A Lindsey-Boltz
View Article
humanWB

Cell Rep

Pharmacological inhibition of neddylation impairs long interspersed element 1 retrotransposition

Authors - Yan Li
  • KD Validated
View Article