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RNASE11 Polyclonal antibody

RNASE11 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17203-1-AP

Synonyms

C14orf6, Probable ribonuclease 11, RNase 11, RNASE11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNASE11 Polyclonal antibody (17203-1-AP)

    WB analysis using 17203-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17203-1-AP (RNASE11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using RNASE11 Polyclonal antibody (17203-1-AP)

    WB analysis of human testis using 17203-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 17203-1-AP (RNASE11 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using RNASE11 Polyclonal antibody (17203-1-AP)

    IHC staining of human gliomas using 17203-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17203-1-AP (RNASE11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using RNASE11 Polyclonal antibody (17203-1-AP)

    IHC staining of human gliomas using 17203-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17203-1-AP (RNASE11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inL02 cells, human testis tissue, HepG2 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17203-1-AP targets RNASE11 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNASE11 fusion protein Ag10981
Full Name ribonuclease, RNase A family, 11 (non-active)
Calculated Molecular Weight 199 aa, 22 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC025410
Gene Symbol RNASE11
Gene ID (NCBI) 122651
RRIDAB_2253967
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNASE11, also named as C14ORF6, is ribonuclease 11. This antibody detected 30 kDa band with Testis and L02 cell lysate.

Protocols

Product Specific Protocols
WB protocol for RNASE11 antibody 17203-1-APDownload protocol
IHC protocol for RNASE11 antibody 17203-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols