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RLBP1 Polyclonal antibody

RLBP1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 15356-1-AP

Synonyms

Retinaldehyde-binding protein 1, CRALBP, Cellular retinaldehyde-binding protein

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using RLBP1 Polyclonal antibody (15356-1-AP)

    WB analysis using 15356-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15356-1-AP (RLBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RLBP1 Polyclonal antibody (15356-1-AP)

    WB analysis of HepG2 using 15356-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15356-1-AP (RLBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human eye tissue using RLBP1 Polyclonal antibody (15356-1-AP)

    IHC staining of human eye using 15356-1-AP

    Immunohistochemical analysis of paraffin-embedded human eye using 15356-1-AP (RLBP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human eye tissue using RLBP1 Polyclonal antibody (15356-1-AP)

    IHC staining of human eye using 15356-1-AP

    Immunohistochemical analysis of paraffin-embedded human eye using 15356-1-AP (RLBP1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using RLBP1 Polyclonal antibody (15356-1-AP)

    IHC staining of mouse eye using 15356-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 15356-1-AP (RLBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using RLBP1 Polyclonal antibody (15356-1-AP)

    IHC staining of mouse eye using 15356-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 15356-1-AP (RLBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using RLBP1 Polyclonal antibody (15356-1-AP)

    IF Staining of mouse eye using 15356-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using RLBP1 antibody (15356-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using RLBP1 Polyclonal antibody (15356-1-AP)

    IF Staining of mouse eye using 15356-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using RLBP1 antibody (15356-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RLBP1 Polyclonal antibody (15356-1-AP)

    IF Staining of HeLa using 15356-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RLBP1 antibody (15356-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of Y79 cells using RLBP1 Polyclonal antibody (15356-1-AP)

    IF Staining of Y79 using 15356-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Y79 cells using RLBP1 antibody (15356-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of Retinal organoids using RLBP1 Polyclonal antibody (15356-1-AP)

    IF Staining of Retinal organoids using 15356-1-AP

    Retinal organoids (day 250) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Tubulin beta 3/TUJ1 using 66375-1-Ig at 1:500 dilution (green) and CRALBP using 15356-1-AP at 1:400 (red). Nuclear stain DAPI (blue). Scale bar = 50 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.

Tested Applications

Positive WB detected inmouse eye tissue, HepG2 cells, mouse retina tissue, rat eye tissue
Positive IHC detected inmouse eye tissue, human eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue, Retinal organoids
Positive IF/ICC detected inHeLa cells, Y79 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15356-1-AP targets RLBP1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RLBP1 fusion protein Ag7602
Full Name retinaldehyde binding protein 1
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC004199
Gene Symbol RLBP1
Gene ID (NCBI) 6017
RRIDAB_2178530
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RLBP1 (Retinaldehyde-binding protein 1) is also named as cellular retinaldehyde-binding protein (CRALBP). CRALBP, a 36-kDa aqueous soluble carrier, is expressed in abundance by both retinal pigment epithelial (RPE) and Muller cells of retina and is important for rod- and cone-driven vision (PMID: 32188692). Retinal RLBP1 expression was decreased in diabetes, and its overexpression in Müller glia mitigated DR-associated neurovascular degeneration (PMID: 33674409).

Protocols

Product Specific Protocols
WB protocol for RLBP1 antibody 15356-1-APDownload protocol
IHC protocol for RLBP1 antibody 15356-1-APDownload protocol
IF protocol for RLBP1 antibody 15356-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
zebrafishIF

Aging Cell

Müller Glia maintain their regenerative potential despite degeneration in the aged zebrafish retina.

Authors - Raquel R Martins
View Article
humanWB,IF

Mol Vis

Morphological, biochemical, and transcriptomic characterization of iPSC-derived human RPE cells from normal and Smith-Lemli-Opitz syndrome patients

Authors - Michael H Farkas
View Article
mouseIF

Diabetes

CD40 in Retinal Müller Cells Induces P2X7-Dependent Cytokine Expression in Macrophages/Microglia in Diabetic Mice and Development of Early Experimental Diabetic Retinopathy.

Authors - Jose-Andres C Portillo
View Article
mouseWB

FASEB J

A cell-penetrating CD40-TRAF2,3 blocking peptide diminishes inflammation and neuronal loss after ischemia/reperfusion.

Authors - Jose-Andres C Portillo
View Article
ratIF

iScience

Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration

Authors - Haibin Tian
View Article
humanIF

Invest Ophthalmol Vis Sci

CD40 Upregulation in the Retina of Patients With Diabetic Retinopathy: Association With TRAF2/TRAF6 Upregulation and Inflammatory Molecule Expression

Authors - Sarah Vos
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Ryan (Verified Customer) (01-24-2018)

Antigen retrieval in NaCit 0.1M ph 6

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Retina
RLBP1 Antibody Immunohistochemistry, validation (1:100 dilution) in Retina (Cat no:15356-1-AP)