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RIOK1 Polyclonal antibody

RIOK1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 17222-1-AP

Synonyms

AD034, RIO kinase 1, RIO kinase 1 (yeast), RIOK1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RIOK1 Polyclonal antibody (17222-1-AP)

    WB analysis of HeLa using 17222-1-AP

    WB result of RIOK1 antibody (17222-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RIOK1 transfected HeLa cells.

  • Western Blot (WB) analysis of HeLa cells using RIOK1 Polyclonal antibody (17222-1-AP)

    WB analysis of HeLa using 17222-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17222-1-AP (RIOK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RIOK1 Polyclonal antibody (17222-1-AP)

    WB analysis using 17222-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17222-1-AP (RIOK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using RIOK1 Polyclonal antibody (17222-1-AP)

    IP experiment of HeLa using 17222-1-AP

    IP result of anti-RIOK1 (IP:17222-1-AP, 4ug; Detection:17222-1-AP 1:500) with HeLa cells lysate 3200ug.

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

17222-1-AP targets RIOK1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RIOK1 fusion protein Ag11057
Full Name RIO kinase 1 (yeast)
Calculated Molecular Weight 568 aa, 66 kDa
Observed Molecular Weight 75-80 kDa
GenBank Accession NumberBC006104
Gene Symbol RIOK1
Gene ID (NCBI) 83732
RRIDAB_2284990
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RIOK1 antibody 17222-1-APDownload protocol
IP protocol for RIOK1 antibody 17222-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biomedicines

RIOK2 Inhibitor NSC139021 Exerts Anti-Tumor Effects on Glioblastoma via Inducing Skp2-Mediated Cell Cycle Arrest and Apoptosis.

Authors - Min Yu
View Article
humanWB

iScience

Ras Post-transcriptionally Enhances a Pre-malignantly Primed EMT to Promote Invasion.

Authors - Laura S Bisogno
View Article
humanWB

Cancer Res Treat

Elevated Expression of RIOK1 Is Correlated with Breast Cancer Hormone Receptor Status and Promotes Cancer Progression.

Authors - Zhiqi Huang
View Article