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RINT1 Polyclonal antibody

RINT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14567-1-AP

Synonyms

RINT-1, RINT 1, RAD50-interacting protein 1, HsRINT-1, HsRINT1

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using RINT1 Polyclonal antibody (14567-1-AP)

    WB analysis of HepG2 using 14567-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14567-1-AP (RINT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RINT1 Polyclonal antibody (14567-1-AP)

    WB analysis of HepG2 using 14567-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14567-1-AP (RINT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RINT1 Polyclonal antibody (14567-1-AP)

    WB analysis of HepG2 using 14567-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14567-1-AP (RINT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RINT1 Polyclonal antibody (14567-1-AP)

    IHC staining of human breast cancer using 14567-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14567-1-AP (RINT1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RINT1 Polyclonal antibody (14567-1-AP)

    IHC staining of human breast cancer using 14567-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14567-1-AP (RINT1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RINT1 Polyclonal antibody (14567-1-AP)

    IHC staining of human breast cancer using 14567-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14567-1-AP (RINT1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RINT1 Polyclonal antibody (14567-1-AP)

    IHC staining of human breast cancer using 14567-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14567-1-AP (RINT1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RINT1 Polyclonal antibody (14567-1-AP)

    IHC staining of human liver cancer using 14567-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 14567-1-AP (RINT1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RINT1 Polyclonal antibody (14567-1-AP)

    IHC staining of human liver cancer using 14567-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 14567-1-AP (RINT1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RINT1 Polyclonal antibody (14567-1-AP)

    IF Staining of HepG2 using 14567-1-AP

    Immunofluorescent analysis of HepG2 cells using 14567-1-AP (RINT1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

14567-1-AP targets RINT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RINT1 fusion protein Ag6145
Full Name RAD50 interactor 1
Calculated Molecular Weight 91 kDa
Observed Molecular Weight 87 kDa
GenBank Accession NumberBC068483
Gene Symbol RINT1
Gene ID (NCBI) 60561
RRIDAB_2269421
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RINT-1 was first identified as a Rad50-interacting protein that participates in radiation-induced G2/M checkpoint control. It also involved in regulation of membrane traffic between the endoplasmic reticulum and the Golgi. It interacts with p130, an Rb family protein, and together they regulate the telomerase-independent telomere length. Otherwise, it interacts with Zeste white 10 with its N-terminal region throughout the cell cycle.

Protocols

Product Specific Protocols
WB protocol for RINT1 antibody 14567-1-APDownload protocol
IHC protocol for RINT1 antibody 14567-1-APDownload protocol
IF protocol for RINT1 antibody 14567-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

J Biol Chem

Comparative proximity biotinylation implicates the small GTPase RAB18 in sterol mobilization and biosynthesis

Authors - Robert S Kiss
View Article