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RIN1 Polyclonal antibody

RIN1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16388-1-AP

Synonyms

Ras interaction/interference protein 1, Ras inhibitor JC99, Ras and Rab interactor 1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RIN1 Polyclonal antibody (16388-1-AP)

    WB analysis of HeLa using 16388-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16388-1-AP (RIN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RIN1 Polyclonal antibody (16388-1-AP)

    WB analysis of HeLa using 16388-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16388-1-AP (RIN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using RIN1 Polyclonal antibody (16388-1-AP)

    IP experiment of HeLa using 16388-1-AP

    IP result of anti-RIN1 (IP:16388-1-AP, 4ug; Detection:16388-1-AP 1:500) with HeLa cells lysate 3400ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RIN1 Polyclonal antibody (16388-1-AP)

    IF Staining of HeLa using 16388-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16388-1-AP (RIN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16388-1-AP targets RIN1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RIN1 fusion protein Ag9451
Full Name Ras and Rab interactor 1
Calculated Molecular Weight 783 aa, 84 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC014417
Gene Symbol RIN1
Gene ID (NCBI) 9610
RRIDAB_2177839
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RIN1 antibody 16388-1-APDownload protocol
IF protocol for RIN1 antibody 16388-1-APDownload protocol
IP protocol for RIN1 antibody 16388-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Comput Struct Biotechnol J

Structure-based discovery of neoandrographolide as a novel inhibitor of Rab5 to suppress cancer growth.

Authors - Jing Zhang
View Article
humanIF

Cell Commun Signal

Circular dorsal ruffles disturb the growth factor-induced PI3K-AKT pathway in hepatocellular carcinoma Hep3B cells.

Authors - Xiaowei Sun
View Article