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  • Featured Product
  • KD/KO Validated

RICTOR Polyclonal antibody

RICTOR Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 27248-1-AP

Synonyms

Rapamycin-insensitive companion of mTOR, KIAA1999, hAVO3, AVO3 homolog

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RICTOR Polyclonal antibody (27248-1-AP)

    WB analysis using 27248-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27248-1-AP (RICTOR antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RICTOR Polyclonal antibody (27248-1-AP)

    WB analysis using 27248-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27248-1-AP (RICTOR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using RICTOR Polyclonal antibody (27248-1-AP)

    WB analysis of NIH/3T3 using 27248-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27248-1-AP (RICTOR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RICTOR Polyclonal antibody (27248-1-AP)

    IHC staining of human lung cancer using 27248-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27248-1-AP (RICTOR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RICTOR Polyclonal antibody (27248-1-AP)

    IHC staining of human lung cancer using 27248-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27248-1-AP (RICTOR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RICTOR Polyclonal antibody (27248-1-AP)

    IHC staining of mouse testis using 27248-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27248-1-AP (RICTOR antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RICTOR Polyclonal antibody (27248-1-AP)

    IHC staining of mouse testis using 27248-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27248-1-AP (RICTOR antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RICTOR Polyclonal antibody (27248-1-AP)

    IF Staining of HeLa using 27248-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RICTOR antibody (27248-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using RICTOR Polyclonal antibody (27248-1-AP)

    IF Staining of NIH/3T3 using 27248-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RICTOR antibody (27248-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RICTOR Polyclonal antibody (27248-1-AP)

    IF Staining of HeLa using 27248-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 27248-1-AP (RICTOR antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, NIH/3T3 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman lung cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27248-1-AP targets RICTOR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RICTOR fusion protein Ag25649
Full Name rapamycin-insensitive companion of mTOR
Calculated Molecular Weight 192 kDa
Observed Molecular Weight 192 kDa
GenBank Accession NumberBC029608
Gene Symbol RICTOR
Gene ID (NCBI) 253260
RRIDAB_2880817
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RICTOR antibody 27248-1-APDownload protocol
IHC protocol for RICTOR antibody 27248-1-APDownload protocol
IF protocol for RICTOR antibody 27248-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Neurosci

A glycolytic shift in Schwann cells supports injured axons.

Authors - Elisabetta Babetto
View Article
humanWB

Aging (Albany NY)

Interleukin 6 promotes BMP9-induced osteoblastic differentiation through Stat3/mTORC1 in mouse embryonic fibroblasts

Authors - Shi-Yu Wang
View Article
mouseWB,IF

Cell Rep

Mitochondrial dynamics define muscle fiber type by modulating cellular metabolic pathways

Authors - Tatsuki Yasuda
View Article
mouseIF

J Cell Physiol

Identification of circular dorsal ruffles as signal platforms for the AKT pathway in glomerular podocytes

Authors - Rui Hua
View Article
humanWB

J Ethnopharmacol

Diterpenoid tanshinones inhibit gastric cancer angiogenesis through the PI3K/Akt/mTOR signaling pathway

Authors - Jie-Ru Yu
View Article
humanWB

Cell Commun Signal

Rapamycin promotes endothelial-mesenchymal transition during stress-induced premature senescence through the activation of autophagy.

Authors - Norihiko Sasaki
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Joleen (Verified Customer) (06-12-2019)

The antibody recognizes RICTOR which is around ~200kD. It is the top most band. The first two lanes were cell lysates and show that the antibody recognizes other proteins non-specifically. The last lane contained eluted proteins that are presumably proximal to the cell membrane. RICTOR shows up there cleanly.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
RICTOR Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:27248-1-AP)