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RIC8A Polyclonal antibody

RIC8A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18707-1-AP

Synonyms

Synembryn-A, Synembryn A, synembryn, RIC8, Protein Ric-8A

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using RIC8A Polyclonal antibody (18707-1-AP)

    WB analysis of human brain using 18707-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 18707-1-AP (RIC8A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using RIC8A Polyclonal antibody (18707-1-AP)

    IHC staining of human brain using 18707-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 18707-1-AP (RIC8A Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using RIC8A Polyclonal antibody (18707-1-AP)

    IHC staining of human brain using 18707-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 18707-1-AP (RIC8A Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RIC8A Polyclonal antibody (18707-1-AP)

    IHC staining of human pancreas cancer using 18707-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 18707-1-AP (RIC8A Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RIC8A Polyclonal antibody (18707-1-AP)

    IHC staining of human pancreas cancer using 18707-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 18707-1-AP (RIC8A Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RIC8A Polyclonal antibody (18707-1-AP)

    IF Staining of HeLa using 18707-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 18707-1-AP (RIC8A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman brain tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

18707-1-AP targets RIC8A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name resistance to inhibitors of cholinesterase 8 homolog A (C. elegans)
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC011821
Gene Symbol RIC8A
Gene ID (NCBI) 60626
RRIDAB_2179831
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RIC8A, also named as synembryn-A, is a Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins by exchanging bound GDP for free GTP. It is involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. RIC8A also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.

Protocols

Product Specific Protocols
WB protocol for RIC8A antibody 18707-1-APDownload protocol
IHC protocol for RIC8A antibody 18707-1-APDownload protocol
IF protocol for RIC8A antibody 18707-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Dev Dyn

Nucleotide exchange factor RIC-8 is indispensable in mammalian early development.

Authors - T?nissoo Tambet T
View Article