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RIC8A Polyclonal antibody

RIC8A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11138-1-AP

Synonyms

Protein Ric 8A, RIC8, RIC8A, synembryn, Synembryn A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RIC8A Polyclonal antibody (11138-1-AP)

    WB analysis using 11138-1-AP

    BxPC-3 cells and Jurkat cells were subjected to SDS PAGE followed by western blot with 11138-1-AP (RIC8A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using RIC8A Polyclonal antibody (11138-1-AP)

    WB analysis of human brain using 11138-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11138-1-AP (RIC8A antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RIC8A Polyclonal antibody (11138-1-AP)

    IHC staining of human pancreas cancer using 11138-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11138-1-AP (RIC8A antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inBxPC-3 cells, human brain tissue, Jurkat cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11138-1-AP targets RIC8A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RIC8A fusion protein Ag1593
Full Name resistance to inhibitors of cholinesterase 8 homolog A (C. elegans)
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 58-62 kDa
GenBank Accession NumberBC011821
Gene Symbol RIC8A
Gene ID (NCBI) 60626
RRIDAB_2179822
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RIC8A, also named as synembryn-A, is a Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins by exchanging bound GDP for free GTP. It is involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. RIC8A also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.

Protocols

Product Specific Protocols
WB protocol for RIC8A antibody 11138-1-APDownload protocol
IHC protocol for RIC8A antibody 11138-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proc Natl Acad Sci U S A

CYLD regulates spindle orientation by stabilizing astral microtubules and promoting dishevelled-NuMA-dynein/dynactin complex formation.

Authors - Yunfan Yang
View Article
mouseWB,IHC

Front Cell Neurosci

Ric-8A, a Galpha protein guanine nucleotide exchange factor potentiates taste receptor signaling.

Authors - Fenech Claire C
View Article