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  • Featured Product
  • KD/KO Validated

RHPN2 Polyclonal antibody

RHPN2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12671-1-AP

Synonyms

Rhophilin-2, Rhophilin 2, RhoBP, p76RBE, GTP-Rho-binding protein 2

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using RHPN2 Polyclonal antibody (12671-1-AP)

    WB analysis of COLO 320 using 12671-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 12671-1-AP (RHPN2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RHPN2 Polyclonal antibody (12671-1-AP)

    WB analysis of HepG2 using 12671-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12671-1-AP (RHPN2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of COLO 320 cells using RHPN2 Polyclonal antibody (12671-1-AP)

    WB analysis of COLO 320 using 12671-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 12671-1-AP (RHPN2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RHPN2 Polyclonal antibody (12671-1-AP)

    WB analysis of HepG2 using 12671-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12671-1-AP (RHPN2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using RHPN2 Polyclonal antibody (12671-1-AP)

    IHC staining of human colon using 12671-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12671-1-AP (RHPN2 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using RHPN2 Polyclonal antibody (12671-1-AP)

    IHC staining of human colon using 12671-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12671-1-AP (RHPN2 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using RHPN2 Polyclonal antibody (12671-1-AP)

    IHC staining of human stomach using 12671-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12671-1-AP (RHPN2 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using RHPN2 Polyclonal antibody (12671-1-AP)

    IHC staining of human stomach using 12671-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12671-1-AP (RHPN2 antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RHPN2 Polyclonal antibody (12671-1-AP)

    IF Staining of HepG2 using 12671-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RHPN2 antibody (12671-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

Tested Applications

Positive WB detected inCOLO 320 cells, HepG2 cells
Positive IHC detected inhuman colon tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12671-1-AP targets RHPN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RHPN2 fusion protein Ag3353
Full Name rhophilin, Rho GTPase binding protein 2
Calculated Molecular Weight 686 aa, 77 kDa
Observed Molecular Weight 77 kDa
GenBank Accession NumberBC036447
Gene Symbol RHPN2
Gene ID (NCBI) 85415
RRIDAB_2877872
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RHPN2 binds specifically to GTP-Rho. It may function in a Rho pathway to limit stress fiber formation and/or increase the turnover of F-actin structures in the absence of high levels of RhoA activity. The MW of this protein is 77 kDa, and this antibody specially recognises the 77 kDa protein.

Protocols

Product Specific Protocols
WB protocol for RHPN2 antibody 12671-1-APDownload protocol
IHC protocol for RHPN2 antibody 12671-1-APDownload protocol
IF protocol for RHPN2 antibody 12671-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Sci Signal

RhoA inhibits neural differentiation in murine stem cells through multiple mechanisms.

Authors - Junning Yang
View Article
humanWB,IF

Cell Cycle

P76RBE silencing inhibits ovarian cancer cell proliferation, migration, and invasion via suppressing the integrin β1/NF-κB pathway.

Authors - Limei Yan
  • KD Validated
View Article
mouseWB

J Biochem Mol Toxicol

Circ_0000284 upregulates RHPN2 to facilitate pancreatic cancer proliferation, metastasis, and angiogenesis through sponging miR-1179

Authors - Jian Zhang
View Article