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  • Featured Product
  • KD/KO Validated

RHOV Polyclonal antibody

RHOV Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26620-1-AP

Synonyms

ARHV, CDC42 like GTPase 2, Chp, GTP binding protein like 2, Rho GTPase like protein ARHV, RHOV, WRCH 2, WRCH2

Validation Data Gallery

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  • Western Blot (WB) analysis of rat lung tissue using RHOV Polyclonal antibody (26620-1-AP)

    WB analysis of rat lung using 26620-1-AP

    rat lung tissue were subjected to SDS PAGE followed by western blot with 26620-1-AP (RHOV Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RHOV Polyclonal antibody (26620-1-AP)

    IHC staining of human lung cancer using 26620-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26620-1-AP (RHOV Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RHOV Polyclonal antibody (26620-1-AP)

    IHC staining of human lung cancer using 26620-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26620-1-AP (RHOV Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inrat lung tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26620-1-AP targets RHOV in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RHOV fusion protein Ag24331
Full Name ras homolog gene family, member V
Calculated Molecular Weight 236 aa, 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC112945
Gene Symbol RHOV
Gene ID (NCBI) 171177
RRIDAB_2880576
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RHOV antibody 26620-1-APDownload protocol
IHC protocol for RHOV antibody 26620-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Biol Sci

RHOV promotes lung adenocarcinoma cell growth and metastasis through JNK/c-Jun pathway.

Authors - Deyu Zhang
View Article
humanWB,IHC

Front Oncol

Overexpression of RhoV Promotes the Progression and EGFR-TKI Resistance of Lung Adenocarcinoma.

Authors - Hongjin Chen
  • KD Validated
View Article