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RHOF Polyclonal antibody

RHOF Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 12290-1-AP

Synonyms

ARHF, Rho family GTPase Rif, Rho in filopodia, RHOF, RIF

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using RHOF Polyclonal antibody (12290-1-AP)

    WB analysis using 12290-1-AP

    Various cells were subjected to SDS PAGE followed by western blot with 12290-1-AP (RHOF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RHOF Polyclonal antibody (12290-1-AP)

    WB analysis using 12290-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12290-1-AP (RHOF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using RHOF Polyclonal antibody (12290-1-AP)

    WB analysis of human brain using 12290-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12290-1-AP (RHOF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using RHOF Polyclonal antibody (12290-1-AP)

    WB analysis of COLO 320 using 12290-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 12290-1-AP (RHOF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of COLO 320 cells using RHOF Polyclonal antibody (12290-1-AP)

    IP experiment of COLO 320 using 12290-1-AP

    IP result of anti-RHOF (IP:12290-1-AP, 4ug; Detection:12290-1-AP 1:300) with COLO 320 cells lysate 2400 ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RHOF Polyclonal antibody (12290-1-AP)

    IHC staining of human colon cancer using 12290-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RHOF Polyclonal antibody (12290-1-AP)

    IHC staining of human colon cancer using 12290-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using RHOF Polyclonal antibody (12290-1-AP)

    IHC staining of human small intestine using 12290-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using RHOF Polyclonal antibody (12290-1-AP)

    IHC staining of human small intestine using 12290-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using RHOF Polyclonal antibody (12290-1-AP)

    IHC staining of human small intestine using 12290-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using RHOF Polyclonal antibody (12290-1-AP)

    IHC staining of human small intestine using 12290-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RHOF Polyclonal antibody (12290-1-AP)

    IHC staining of human colon cancer using 12290-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RHOF Polyclonal antibody (12290-1-AP)

    IHC staining of human colon cancer using 12290-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inA549 cells, HeLa cells, human brain tissue, COLO 320 cells, mouse brain tissue, rat brain tissue
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman colon cancer tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12290-1-AP targets RHOF in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RHOF fusion protein Ag2943
Full Name ras homolog gene family, member F (in filopodia)
Calculated Molecular Weight 211 aa, 24 kDa
Observed Molecular Weight24 kDa
GenBank Accession NumberBC018208
Gene Symbol RHOF
Gene ID (NCBI) 54509
RRIDAB_10644126
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RHOF antibody 12290-1-APDownload protocol
IHC protocol for RHOF antibody 12290-1-APDownload protocol
IP protocol for RHOF antibody 12290-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

Rho family small GTPase Rif regulates Wnt5a-Ror1-Dvl2 signaling and promotes lung adenocarcinoma progression

Authors - Michiru Nishita
View Article
humanWB

Int J Mol Sci

An Integrated Multi-Omics Analysis Identifying Immune Subtypes of Pancreatic Cancer

Authors - Yongcheng Su
View Article
human,mouseWB,IHC,IF

iScience

RHOF activation of AKT/β-catenin signaling pathway drives acute myeloid leukemia progression and chemotherapy resistance

Authors - Xin Wen
  • KD Validated
View Article