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RGSL1 Polyclonal antibody

RGSL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25836-1-AP

Synonyms

RGSL, RGSL1, RGSL2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using RGSL1 Polyclonal antibody (25836-1-AP)

    WB analysis of mouse brain using 25836-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25836-1-AP (RGSL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using RGSL1 Polyclonal antibody (25836-1-AP)

    IHC staining of human testis using 25836-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25836-1-AP (RGSL1 Antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using RGSL1 Polyclonal antibody (25836-1-AP)

    IHC staining of human testis using 25836-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25836-1-AP (RGSL1 Antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25836-1-AP is RGSL1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RGSL1 fusion protein Ag20269
Full Name regulator of G-protein signaling like 1
Calculated Molecular Weight 1076 aa, 126 kDa
Observed Molecular Weight 125 kDa
GenBank Accession NumberBC142944
Gene Symbol RGSL1
Gene ID (NCBI) 353299
RRIDAB_2880263
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RGSL1, also named as RGSL and RGSL2, belongs to the RSG family. RGSL1 is involved in the G-protein signaling regulation with a suggested role in prostate carcinogenesis (PMID: 28662289). RGSL1 has 6 isoforms with the molecular mass of 47-63, 71 and 126 kDa. RGSL1 may interact with other markers in the development of breast cancer (PMID: 21135262).

Protocols

Product Specific Protocols
WB protocol for RGSL1 antibody 25836-1-APDownload protocol
IHC protocol for RGSL1 antibody 25836-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols