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  • Featured Product
  • KD/KO Validated

REEP5 Polyclonal antibody

REEP5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14643-1-AP

Synonyms

TB2, Receptor expression-enhancing protein 5, Protein TB2, Polyposis locus protein 1, DP1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using REEP5 Polyclonal antibody (14643-1-AP)

    WB analysis using 14643-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14643-1-AP (REEP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using REEP5 Polyclonal antibody (14643-1-AP)

    WB analysis of HeLa using 14643-1-AP

    WB result of REEP5 antibody (14643-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-REEP5 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using REEP5 Polyclonal antibody (14643-1-AP)

    WB analysis using 14643-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14643-1-AP (REEP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using REEP5 Polyclonal antibody (14643-1-AP)

    WB analysis of rat brain using 14643-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 14643-1-AP (REEP5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using REEP5 Polyclonal antibody (14643-1-AP)

    WB analysis of mouse brain using 14643-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14643-1-AP (REEP5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using REEP5 Polyclonal antibody (14643-1-AP)

    WB analysis of human liver using 14643-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 14643-1-AP (REEP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using REEP5 Polyclonal antibody (14643-1-AP)

    IP experiment of HeLa using 14643-1-AP

    IP result of anti-REEP5 (IP:14643-1-AP, 3ug; Detection:14643-1-AP 1:1000) with HeLa cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using REEP5 Polyclonal antibody (14643-1-AP)

    IHC staining of human colon cancer using 14643-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14643-1-AP (REEP5 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using REEP5 Polyclonal antibody (14643-1-AP)

    IHC staining of human colon cancer using 14643-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14643-1-AP (REEP5 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using REEP5 Polyclonal antibody (14643-1-AP)

    IF Staining of A549 using 14643-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using REEP5 antibody (14643-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using REEP5 Polyclonal antibody (14643-1-AP)

    IF Staining of HeLa using 14643-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14643-1-AP (REEP5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, human liver tissue, mouse brain tissue, rat brain tissue, mouse heart tissue, rat heart tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14643-1-AP targets REEP5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, monkey, xenopus
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen REEP5 fusion protein Ag6229
Full Name receptor accessory protein 5
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 18-21 kDa
GenBank Accession NumberBC065926
Gene Symbol REEP5
Gene ID (NCBI) 7905
RRIDAB_2178440
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

REEP5 (receptor expression-enhancing protein 5), also named as C5orf18, DP1 or TB2, is a membrane protein belonging to DP1/Yop1p protein family. All REEP proteins contain a conserved TB2/DP1, HVA22 domain which is involved in intracellular trafficking and secretion (PMID: 19123125). REEP5 is expressed in circumvallate papillae and testis, and may have the function to influence functional cell surface expression of taste receptors (PMID: 16720576). REEP5 can interact with atlastins (ATL1 and ATL2) which are involved in modulating the structure of ER network (PMID: 19665976).

Protocols

Product Specific Protocols
WB protocol for REEP5 antibody 14643-1-APDownload protocol
IHC protocol for REEP5 antibody 14643-1-APDownload protocol
IF protocol for REEP5 antibody 14643-1-APDownload protocol
IP protocol for REEP5 antibody 14643-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

ER proteins decipher the tubulin code to regulate organelle distribution

Authors - Pengli Zheng
View Article
humanWB

Cell

A Genome-wide ER-phagy Screen Highlights Key Roles of Mitochondrial Metabolism and ER-Resident UFMylation.

Authors - Jin Rui Liang
  • KO Validated
View Article
mouseWB

Cell Metab

Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.

Authors - Xiao Wang
View Article
humanWB

Nat Cell Biol

Combinatorial selective ER-phagy remodels the ER during neurogenesis

Authors - Melissa J Hoyer
View Article
human,mouseWB

Cell Res

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Authors - Pengli Zheng
View Article
mouseIP

Mol Psychiatry

Dysfunctional tubular endoplasmic reticulum constitutes a pathological feature of Alzheimer's disease.

Authors - M G Sharoar
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Andrea (Verified Customer) (05-10-2022)

Sharp and strong signal at 21 kDa with clear background.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: HeLa cell line
REEP5 Antibody Western Blot validation (1:250 dilution) in HeLa cell line (Cat no:14643-1-AP)
FH

Christine (Verified Customer) (11-10-2021)

Very good detection of endogenous REEP5 protein in human HEK293T and HeLa cell lines. Band at about 20 kDa (expected size for REEP5)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Cell culture of HeLa and HEK293T
FH

Wei (Verified Customer) (09-22-2020)

The antibody gets sharp, single band on western blot.

  • Applications: Western Blot
  • Cell Tissue Type: Mouse heart
FH

WEI (Verified Customer) (06-29-2020)

The staining pattern is consistent with cardiomyocytes like ER localization.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: Mouse Heart
FH

H (Verified Customer) (04-05-2020)

The antibody is very sensitive, such that 1 hour incubation using 1:3000 dilution worked perfectly.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: HEK293T and HCT116
REEP5 Antibody Western Blot validation (1:3000 dilution) in HEK293T and HCT116 (Cat no:14643-1-AP)