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Radixin Polyclonal antibody

Radixin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13790-1-AP

Synonyms

RDX

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse pancreas tissue using Radixin Polyclonal antibody (13790-1-AP)

    WB analysis of mouse pancreas using 13790-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13790-1-AP (Radixin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using Radixin Polyclonal antibody (13790-1-AP)

    WB analysis of Y79 using 13790-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 13790-1-AP (Radixin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using Radixin Polyclonal antibody (13790-1-AP)

    WB analysis of HEK-293 using 13790-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13790-1-AP (Radixin antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Radixin Polyclonal antibody (13790-1-AP)

    IHC staining of human pancreas cancer using 13790-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13790-1-AP (Radixin antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Radixin Polyclonal antibody (13790-1-AP)

    IHC staining of human pancreas cancer using 13790-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13790-1-AP (Radixin antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Radixin Polyclonal antibody (13790-1-AP)

    IHC staining of human pancreas cancer using 13790-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13790-1-AP (Radixin antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Radixin Polyclonal antibody (13790-1-AP)

    IHC staining of human pancreas cancer using 13790-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13790-1-AP (Radixin antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Radixin Polyclonal antibody (13790-1-AP)

    IHC staining of human pancreas using 13790-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 13790-1-AP (Radixin antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Radixin Polyclonal antibody (13790-1-AP)

    IHC staining of human pancreas using 13790-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 13790-1-AP (Radixin antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Radixin Polyclonal antibody (13790-1-AP)

    IHC staining of human liver cancer using 13790-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 13790-1-AP (Radixin antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Radixin Polyclonal antibody (13790-1-AP)

    IHC staining of human liver cancer using 13790-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 13790-1-AP (Radixin antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Radixin Polyclonal antibody (13790-1-AP)

    IF Staining of MCF-7 using 13790-1-AP

    Immunofluorescent analysis of MCF-7 cells using 13790-1-AP (Radixin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse pancreas tissue, HEK-293 cells, Y79 cells
Positive IHC detected inhuman pancreas cancer tissue, human liver cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13790-1-AP targets Radixin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Radixin fusion protein Ag4813
Full Name radixin
Calculated Molecular Weight 583 aa, 68 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC047109
Gene Symbol RDX
Gene ID (NCBI) 5962
RRIDAB_2300889
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Radixin belongs to the ezrin-radixin-moesin (ERM) family of proteins which act as cross-linkers between membrane and actin cytoskeleton. ERM proteins provide structural links to strengthen the cell cortex and facilitate several key cellular processes, including membrane dynamics, substrate adhesion, cell survival, cell adhesion, and motility. The function of ERM proteins is highly reliant on phosphorylation induced conformational changes in response to chemokine and antigen stimulation.

Protocols

Product Specific Protocols
WB protocol for Radixin antibody 13790-1-APDownload protocol
IHC protocol for Radixin antibody 13790-1-APDownload protocol
IF protocol for Radixin antibody 13790-1-APDownload protocol
FC protocol for Radixin antibody 13790-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Am J Pathol

Elevated Expression of Moesin in Muscular Dystrophies.

Authors - Mark Pines
View Article
humanWB

Drug Resist Updat

Intercellular adhesion molecule-1 suppresses TMZ chemosensitivity in acquired TMZ-resistant gliomas by increasing assembly of ABCB1 on the membrane

Authors - Xin Zhang
View Article