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NELFE Polyclonal antibody
NELFE Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ChIP, RIP, ELISA
Conjugate
Unconjugated
Cat no : 10705-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, mouse brain tissue, mouse eye tissue, mouse placenta tissue, mouse kidney tissue, rat kidney tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 16 publications below |
| IHC | See 4 publications below |
| IF | See 2 publications below |
| ChIP | See 4 publications below |
| RIP | See 1 publications below |
Product Information
10705-1-AP targets NELFE in WB, IHC, IF/ICC, FC (Intra), IP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NELFE fusion protein Ag1186 |
| Full Name | RD RNA binding protein |
| Calculated Molecular Weight | 37 kDa, 43 kDa |
| Observed Molecular Weight | 43 kDa |
| GenBank Accession Number | BC011600 |
| Gene Symbol | NELFE |
| Gene ID (NCBI) | 7936 |
| RRID | AB_513966 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RDBP, also named NELF-E and RD, belongs to the RRM NELF-E family. It is an essential component of the NELF complex which is a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, a complex acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. RDBP was recently found implicated in RNA polymerase II pausing by association with Hsp90. Catalog# 10705-1-AP is a widely used rabbit polyclonal antibody obtained by immunizing animals with full-length RDBP of human origin.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NELFE antibody 10705-1-AP | Download protocol |
| IHC protocol for NELFE antibody 10705-1-AP | Download protocol |
| IF protocol for NELFE antibody 10705-1-AP | Download protocol |
| IP protocol for NELFE antibody 10705-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Hsp90 globally targets paused RNA polymerase to regulate gene expression in response to environmental stimuli. | ||
Nat Commun Negative elongation factor complex enables macrophage inflammatory responses by controlling anti-inflammatory gene expression. | ||
Nat Commun RNA polymerase II pausing factor NELF in CD8+ T cells promotes antitumor immunity. | ||
Dev Cell Negative elongation factor regulates muscle progenitor expansion for efficient myofiber repair and stem cell pool repopulation. | ||
Proc Natl Acad Sci U S A Immediate mediators of the inflammatory response are poised for gene activation through RNA polymerase II stalling. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH HUAI-CHIN (Verified Customer) (02-14-2019) | This antibody works great in Western. Gives me clean single band.
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