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RBMS1 Polyclonal antibody

RBMS1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 11061-2-AP

Synonyms

MSSP 3, MSSP 2, MSSP 1, MSSP, HCC 4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBMS1 Polyclonal antibody (11061-2-AP)

    WB analysis using 11061-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11061-2-AP (RBMS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RBMS1 Polyclonal antibody (11061-2-AP)

    WB analysis of HeLa using 11061-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11061-2-AP (RBMS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RBMS1 Polyclonal antibody (11061-2-AP)

    WB analysis of HeLa using 11061-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11061-2-AP (RBMS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RBMS1 Polyclonal antibody (11061-2-AP)

    WB analysis of HeLa using 11061-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11061-2-AP (RBMS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using RBMS1 Polyclonal antibody (11061-2-AP)

    IP experiment of HeLa using 11061-2-AP

    IP result of anti-RBMS1 (IP:11061-2-AP, 4ug; Detection:11061-2-AP 1:500) with HeLa cells lysate 1200ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RBMS1 Polyclonal antibody (11061-2-AP)

    IF Staining of HepG2 using 11061-2-AP

    Immunofluorescent analysis of HepG2 cells, using RBMS1 antibody 11061-2-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, C6 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11061-2-AP targets RBMS1 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBMS1 fusion protein Ag1493
Full Name RNA binding motif, single stranded interacting protein 1
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC018951
Gene Symbol RBMS1
Gene ID (NCBI) 5937
RRIDAB_2179121
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RBMS1 antibody 11061-2-APDownload protocol
IF protocol for RBMS1 antibody 11061-2-APDownload protocol
IP protocol for RBMS1 antibody 11061-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Epigenetics

Suppressing circIDE/miR-19b-3p/RBMS1 axis exhibits promoting-tumour activity through upregulating GPX4 to diminish ferroptosis in hepatocellular carcinoma

Authors - Hang Zhai
View Article
humanRIP

Mol Cell Biol

Increased ANKRD1 Levels in Early Senescence Mediated by RBMS1-Elicited ANKRD1 mRNA Stabilization

Authors - Chang Hoon Shin
View Article