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RBM6 Polyclonal antibody

RBM6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14360-1-AP

Synonyms

NY LU 12, Lung cancer antigen NY-LU-12, HLC 11, g16, DEF3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBM6 Polyclonal antibody (14360-1-AP)

    WB analysis using 14360-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14360-1-AP (RBM6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using RBM6 Polyclonal antibody (14360-1-AP)

    WB analysis of mouse spleen using 14360-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14360-1-AP (RBM6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using RBM6 Polyclonal antibody (14360-1-AP)

    IP experiment of HeLa using 14360-1-AP

    IP result of anti-RBM6 (IP:14360-1-AP, 4ug; Detection:14360-1-AP 1:500) with HeLa cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RBM6 Polyclonal antibody (14360-1-AP)

    IHC staining of human pancreas cancer using 14360-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14360-1-AP (RBM6 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RBM6 Polyclonal antibody (14360-1-AP)

    IF Staining of HeLa using 14360-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14360-1-AP (RBM6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, mouse spleen tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

14360-1-AP targets RBM6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBM6 fusion protein Ag5743
Full Name RNA binding motif protein 6
Calculated Molecular Weight 129 kDa
Observed Molecular Weight 129 kDa
GenBank Accession NumberBC046643
Gene Symbol RBM6
Gene ID (NCBI) 10180
RRIDAB_2301175
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBM6, also named as DEF3, is a 1123 amino acid protein, which may interact with FAM168B. RBM6 is expressed ubiquitously in adults tissues. RBM6 specifically binds poly(G) RNA homopolymers in vitro. RBM6 exists some isoforms with MV 129 kDa, 69 kDa, and 59 kDa. onversely, fetal thymus and adult kidney express very little RBM6. In the hemopoietic system of mice, the expression of RBM6 is downregulated upon differentiation of progenitor cells into granulocytes but persists during macrophage development.

Protocols

Product Specific Protocols
WB protocol for RBM6 antibody 14360-1-APDownload protocol
IHC protocol for RBM6 antibody 14360-1-APDownload protocol
IF protocol for RBM6 antibody 14360-1-APDownload protocol
IP protocol for RBM6 antibody 14360-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Proteomics Clin Appl

RNA-Binding Motif Protein 6 is a Candidate Serum Biomarker for Pancreatic Cancer.

Authors - Baojun Duan
View Article